A root-knot nematode biocontrol agent and its production process

A technology for biocontrol fungicides and root-knot nematodes, applied in the directions of nematicides, biocides, biocides, etc., to achieve the effects of being beneficial to infection, mycelium germination, and spore growth

Inactive Publication Date: 2011-12-14
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention will provide a root-knot nematode biocontrol fungicide and its production process to overcome the unsatisfactory control effect, poor specificity and poor control effect in the prior art. Problems of short time and high pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A root-knot nematode biocontrol agent is prepared by the following method:

[0021] First prepare Acremonium fungus powder:

[0022] (1) Liquid culture:

[0023] Weigh 2.0 g of colloidal chitin, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0g, calcium chloride (CaCl 2 ) 0.2g, sodium chloride (NaCl) 0.2g, magnesium sulfate (MgSO 4 ) 0.5g, dissolved in 1000ml of distilled water, adjusted to pH 7.0, divided into 500ml conical flasks according to 100ml per bottle, sterilized at 121°C for 30min, inoculated with Acremonium species obtained from the national professional microbial strain library; Said colloidal chitin is prepared by the following method. 10 parts of fine powder chitin is dissolved in 100 parts of concentrated hydrochloric acid, stirred evenly, and placed at 4°C for 24h. Then take 100 parts of concentrated hydrochloric acid and grind it evenly; add 500 parts of 50% ethanol, stir, centrifuge at 4000 r / min for 10 min, remove the supernatant, add distilled w...

Embodiment 2

[0029] A root-knot nematode biocontrol agent is prepared by the following method:

[0030] First prepare Acremonium fungus powder:

[0031] (1) Liquid culture:

[0032] Weigh 1.0 g of colloidal chitin, 1.0 g of glucose, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0g, calcium chloride (CaCl 2 ) 0.2g, sodium chloride (NaCl) 0.2g, magnesium sulfate (MgSO 4 ) 0.5g, dissolved in 1000ml of distilled water, adjusted to pH 7.0, divided into 500ml conical flasks according to 100ml per bottle, sterilized at 121°C for 30min, inoculated with Acremonium biocontrol bacteria, 28°C±1°C, 180rmp vibration amplitude is cultivated for 3 days to make the seed liquid of biocontrol agent, this seed liquid is inoculated in weight ratio by 15% weight volume to be colloidal chitin 7.0g, glucose 7.0g, potassium dihydrogen phosphate (KH 2 PO 4 ) 7.0g, calcium chloride (CaCl 2 ) 1.4g, sodium chloride (NaCl) 1.4g, magnesium sulfate (MgSO 4 ) 3.5g, dissolved in 7000ml of distilled water, adjusted ...

Embodiment 3

[0038] A root-knot nematode biocontrol agent is prepared by the following method:

[0039] First prepare Acremonium fungus powder:

[0040] (1) Liquid culture:

[0041] Weigh 1.5g of colloidal chitin, 0.5g of glucose, potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0g, calcium chloride (CaCl 2 ) 0.2g, sodium chloride (NaCl) 0.2g, magnesium sulfate (MgSO 4 ) 0.5g, dissolved in 1000ml of distilled water, adjusted to pH 7.0, divided into 500ml conical flasks according to 100ml per bottle, sterilized at 121°C for 30min, inoculated with Acremonium biocontrol bacteria, 28°C±1°C, 180rmp vibration amplitude is cultivated for 3 days to make the seed liquid of biocontrol agent, and this seed liquid is inoculated in weight ratio by 15% weight volume to be colloidal chitin 10.5g, glucose 3.5g, potassium dihydrogen phosphate (KH 2 PO 4 ) 7.0g, calcium chloride (CaCl 2 ) 1.4g, sodium chloride (NaCl) 1.4g, magnesium sulfate (MgSO 4 ) 3.5g, dissolved in 7000ml of distilled water, adjust...

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Abstract

The invention belongs to the field of agricultural insect pest biological control, in particular to a root-knot nematode biological control microbial inoculant and a production process thereof. The present invention overcomes the problems of unsatisfactory control effect, poor specificity, short control effect time and large pollution in the prior art. The technical solution of the present invention is: a root-knot nematode biocontrol agent, which is composed of the following raw materials in parts by weight: 10-15 parts of Acremonium fungus powder, 20-30 parts of zeolite, and 50 parts of diatomite -60 parts, 5 parts chitin and 5-10 parts bran. The present invention is a microbial inoculant containing Acremonium spores which have the functions of infecting and killing biological control of root-knot nematodes, and can be used for biological control of serious-harmful root-knot nematode diseases in the process of planting vegetables and fruits in agricultural facilities. The control effect on root-knot nematodes such as peppers, tomatoes and cucumbers can reach more than 93%.

Description

technical field : [0001] The invention belongs to the field of biological control of agricultural insect pests, and in particular relates to a root-knot nematode biological control bacterium and a production process thereof. Background technique : [0002] Root-knot nematode is an obligate endoparasite on plant roots and is the main pathogen that threatens agricultural production worldwide. According to relevant research, root-knot nematode hosts have exceeded 3,000 species of plants, belonging to 114 families, including monocotyledonous plants, dicotyledonous plants, herbaceous plants and gramineous plants. The occurrence of this disease will cause severe crop yield loss, generally resulting in a 10% to 20% yield reduction, and severe cases can reach more than 75%. According to incomplete statistics, nematode damage reduces the world's agricultural output by an average of 24.5% per year, and the economic loss of crops is about 100 billion US dollars. In my country, root...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/16A01N25/08A01P5/00A01G13/00
Inventor 沈卫荣韩丽萍孙晓宇路鹏鹏沈俭陈志杰门欣李玥
Owner MICROBIOLOGY INST OF SHAANXI
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