Method for quantitatively concentrating living cells of Noctiluca scintillans

Abstract

The invention discloses a method for quantitatively concentrating living cells of Noctiluca scintillans. The method is characterized in that: a positioning sleeve with supporting foots is put in a glass ware, the height of the positioning sleeve is lower than the height of the glass ware, the positioning sleeve is covered with a bolting silk, a bottomless collection cylinder is inserted into the positioning sleeve, and the opening of the collection cylinder is higher than the opening of the glass ware, so a simple apparatus which can maintain the suspended concentration of a water sample of the living cells of Noctiluca scintillans is formed; the water sample of the living cells of Noctiluca scintillans is quantitatively and slowly poured into the collection cylinder at a uniform speed, so the living cells of Noctiluca scintillans can be contracted in the collection cylinder, and the shrinking declination of the living cells does not appear; and when the liquid level of the glass wareis stable, the water sample in the collection cylinder can be quantitatively absorbed and can be counted and observed under a microscope. So the method of the invention is a quantitative concentration method with the characteristics of less cell shrinking declination, and maintenance of the suspended contraction and the number of the living cells of Noctiluca scintillans.

Description

technical field [0001] The invention relates to an algae concentration technology, in particular to a method for quantitatively concentrating live cells of nocturnal algae. Background technique [0002] Noctiluca ( Noctiluca scintillans or N. miliaris ), also known as noctiluca, is a heterotrophic unicellular planktonic dinoflagellate species, and the vegetative cells are usually spherical with a diameter of 200 μm to 2000 μm. Noctiluca is a harmful planktonic dinoflagellate species that often causes red tides. Red tides have been reported in sea areas and aquaculture environments around the world. However, the research progress on key issues of noctiluca red tides has been relatively slow. An important reason is However, studies on cell division, cell life history and its significance, and the mechanism of key information substances in the process of population proliferation are quite lacking. Since noctiluca is a completely heterotrophic dinoflagellate, population dyna...

AI Technical Summary

Problems solved by technology

It can be seen that it is far from enough to rely only on the change of fixed cell number in routine monitoring to forecast and control the red tide of Noctiluca nocturna

In the research on the mechanism of nocturnal algae red tide and field monitoring, it is very important to obtain a large number of cell samples before aggregation; it is also found in indoor research that due to the serious floating characteristics of nocturnal algae, the culture water of nocturnal algae cannot form a uniform density suspension. In order to analyze the number of live cells of Nocturnin algae in the culture water, the whole water body can only be counted, which requires a lot of work and takes a long time; moreover, the cells of Noctiluca noctiluca are relatively weak, and excessive disturbance will cause the cells to shrink and decay, which will affect the research results. It is not conducive to research on cell division, cell life history and its significance, and the mechanism of key information substances in the process of population proliferation.

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