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Detecting method for assessing influence of genetically-modified cotton to non-target organisms

A detection method and genetically modified technology, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problem of high cost, achieve low cost, broad application prospects, and reduce labor intensity

Inactive Publication Date: 2013-03-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are limited to a small scale, and the cost is high, which has great limitations in practical applications.

Method used

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  • Detecting method for assessing influence of genetically-modified cotton to non-target organisms
  • Detecting method for assessing influence of genetically-modified cotton to non-target organisms
  • Detecting method for assessing influence of genetically-modified cotton to non-target organisms

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Embodiment

[0024] From July to September 2011, at the Liuhe Cotton Base of Nanjing Institute of Environmental Sciences, we investigated the populations of predators and bollworms in cotton fields of Zhongmian 30 (transgenic Bt cotton) and Zhongmian 16 (parent of Zhongmian 30, non-transgenic cotton) Quantity. Three plots were surveyed for each cotton variety, and the area of ​​each plot was about 2 mu. The beetle survey adopts the method of combining the trap method and the lure method. In each plot, 30 lure cups are arranged according to the chessboard method. The lure cups are plastic cups with smooth inner walls (12 cm high, 9 cm in diameter, and 1 / 4 of the top of the cup wall Drill 4 drainage holes with a diameter of 1.5mm), add 1 part of attractant to each cup, and the attractant is a mixture of vinegar, sugar, alcohol, honey and water. First boil the mixture of sugar: vinegar: 75% alcohol with a mass ratio of 5:4:1 with low heat to make a thick syrup. After boiling, mix 5ml of honey...

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Abstract

The invention belongs to the field of ecological risk assessment of genetically-modified crops and particularly relates to a detecting method for assessing the insect resistance of the transgenic Bt cotton by using chlaenius bioculatus motschulsky. The principle of the method is as follows: the food chain relation of chlaenius bioculatus motschulsky, cotton bollworm and cotton in the cotton field ecosystem is used, and the degree of death caused by the transgenic Bt cotton to the target organism which is the lepidopteron such as the cotton bollworm is reflected by the number of the predator clusters. The chlaenius bioculatus motschulsky is trapped by using the trap and the attractant in the perennially planting transgenic Bt cotton field and the contrast cotton field, and the number of the chlaenius bioculatus motschulsky in the perennially-planting transgenic Bt cotton field is compared with the number of the chlaenius bioculatus motschulsky in the contrast cotton field, and the comparison result shows that the number of the chlaenius bioculatus motschulsky in the perennially-planting transgenic Bt cotton field is lower than the number of the chlaenius bioculatus motschulsky in the contrast cotton field, which proves that the transgenic Bt cotton has higher insect resistance. The detecting method is accurate, is low in cost and is convenient to apply.

Description

technical field [0001] The invention belongs to the field of ecological risk assessment of transgenic crops, and in particular relates to a detection method for evaluating the insect resistance of transgenic Bt gene cotton by using C. bispotae. Background technique [0002] Genetically modified cotton began to be commercially planted on a large scale in 1996, and the global planting area reached 21 million ha by 2010 2 . Transgenic cotton can effectively control cotton field pests, reduce planting costs and increase cotton yield. The target organisms of transgenic Bt cotton are Lepidoptera insects, and a large number of research results show that it has a strong lethal effect on cotton bollworms in a short period of time. However, due to the adaptability of the bollworm, the long-term effects of Bt cotton resistance are unknown. At present, the methods for evaluating the insect resistance of transgenic Bt cotton mainly include leaf-feeding larvae, leaf inoculation wit...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 孙书存吴新卫曹瑞
Owner NANJING UNIV
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