Visual observation culture medium for nematode acute toxicity test and preparation method of visual observation culture medium

Abstract

The invention relates to a visual observation culture medium for nematode acute toxicity test and a preparation method of the visual observation culture medium for the nematode acute toxicity test and aims to solve the problem that an existing culture medium for the nematode acute toxicity test is inaccurate in counting, large in death judgment error, long in observation time and inaccurate in behavior observation. The visual observation culture medium comprises, by mass percentage, 45-60% of gypsum, 5-10% of activated carbon and the balance of distilled water. The preparation method includes uniformly mixing the gypsum with the activated carbon to pour into the distilled water, uniformly stirring to obtain mixture; pouring the mixture into a twelve-hole cell culture plate 0.1-0.2 centimeter in thickness; and standing on a horizontal platform till solidifying and obtaining the visual observation culture medium for the nematode acute toxicity test. The visual observation culture medium is easy to count, obvious in dead nematode judgment, easy for behavior observation and higher in test result correctness and accuracy, and can be applied to the nematode toxicity test.

Description

technical field [0001] The invention relates to a visual observation culture medium for toxicological tests and a preparation method thereof. Background technique [0002] Nematodes, belonging to the phylum Nematoda, are diverse and abundant. They are important ecological factors in ecosystems and are widely used as sensitive indicator organisms for ecological and environmental health assessment. Nematodes can sense changes in the surrounding environment more sensitively, and their life cycle is short, which can indicate environmental changes in a short period of time. Some nematode species that are easy to cultivate and preserve, such as Caenorhabditis elegans, have been widely used as model organisms for research in environmental science, life science and other fields in experimental science such as toxicology. Due to the short life cycle and lifespan of nematodes, nematodes are more often used in acute toxicity tests of 24 hours or less. [0003] The nematode carrier fo...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems of inaccurate base counting, large death judgment error, long observation time and inaccurate behavior observation in the traditional nematode toxicology test culture medium, and provides a nematode acute toxicology test visual observation medium and a preparation method thereof