Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue

A technology for test strips and secreted proteins, which is used in biological testing, material testing, etc. to achieve the effects of good stability, rapid detection and high sensitivity

Inactive Publication Date: 2015-03-25
SHANGHAI TENTH PEOPLES HOSPITAL
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on medical devices for early diagnosis of tongue cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Immunohistochemistry

[0017] Experimental materials: normal tongue tissue, tongue cancer tissue; the primary antibody is goat anti-human CRISP1 antibody, and the secondary antibody is donkey anti-goat Dylight594 antibody.

[0018] experimental method:

[0019] Tissue chip is the orderly combination of a large number of tissue samples on the surface of a micro-substrate, with the help of immunohistochemical method for detection.

[0020] 1. Fix the tissue with PFA fixative for 5 minutes, wash with PBS for 10 minutes

[0021] 2. Antigen retrieval: expose the epitope with 0.01M citrate solution. Microwave on high heat for 3 minutes to heat the repair solution to boiling (4 minutes), then continue to microwave on low heat for 1 minute, twice. Cool to room temperature and wash with PBS for 10 minutes. Membranes were ruptured with 0.5% Triton for 10 minutes.

[0022] 3. Block non-specific proteins

[0023] 1) Soak in PBS for 3 minutes x 3 times

[0024] 2) 3...

Embodiment 2

[0036] Example 2 Enzyme-linked immunosorbent assay (Elisa)

[0037] A total of 90 cases, including 30 cases of tongue cancer (squamous cell carcinoma) case group; 30 cases of other oral diseases (periodontal disease, pulp disease, oral candidiasis) group; 30 cases of healthy control group. The cysteine-rich secreted protein detection kit is used for detection, and the detection steps refer to the instruction manual of the cysteine-rich secreted protein quantitative enzyme-linked detection kit.

[0038] ELISA detection results of CRISP1:

[0039] 1. The tongue cancer group was higher than the other two groups, but there was no difference between the other disease group and the healthy group (P=0.802).

[0040] Table 1 The diagnostic value of salivary CRISP1 in the diagnosis of tongue cancer

[0041] diagnostic index AUC sensitivity specificity false positive rate false negative rate Youden index Urine CRISP1 10 ng / ml 0.809 88.1% 39.3% 60.7% 11....

Embodiment 3

[0044] Example 3 Preparation of gold-labeled antibody complex on test paper

[0045] 1. Colloidal gold preparation

[0046] Add 100ml of 0.005%-0.02% chloroauric acid solution into a round bottom flask and heat to boiling. After boiling, add freshly prepared 0.4%-2% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) 1-2.2ml of aqueous solution, after boiling for 10-25 minutes, continue to stir and cool to room temperature. During this process, it can be seen that the color change of the solution is: golden yellow→black→purple→dark blue→bright red. When the color of the solution completely changes to transparent bright red, the required colloidal gold is obtained. After cooling, put it into a dialysis bag and dialyze it against ultrapure water (1:5000) three times, and finally transfer the dialyzed colloidal gold to a clean glass bottle with a screw cap, and store it in a dark environment at 4°C.

[0047] 2. Connection between gold colloid and protein

[0048] 1. The adsorption...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention relates to an application of CRISP (cysteine-rich secretory protein)1 test paper for diagnosing carcinoma of tongue to preparation of a medical instrument for diagnosing carcinoma of tongue. The test paper is characterized in that a gold colloid pad contains a gold-labelled anti-saliva CRISP1 antibody complex formed by binding colloidal gold and an anti-saliva CRISP1 antibody; the diagnosis sample of the test paper is saliva; and the detection threshold of the test paper is 10-40ng / ml, the concentration of the saliva CRISP1. The test paper is quick in checking, has high sensitivity, strong specificity and good stability, is simple and convenient to operate, dispenses with any instrument, is intuitive and reliable in result judgment, is easy to master and is simultaneously convenient for patients to self-monitor the conditions.

Description

technical field [0001] The present invention relates to the application of the kit, in particular to the application of the CRISP1 kit for diagnosing tongue cancer. Background technique [0002] Tongue cancer can be manifested in three types in the early stage: ulcer, exogenous and infiltrating. In some cases, the first symptom is only tongue pain, which can sometimes be reflected to the temple or ear. The exophytic type can arise from malignant transformation of papilloma. The infiltrative surface may have no protrusions or ulcers. Ulcerative and infiltrating cancers are often accompanied by spontaneous pain and tongue movement limitation of varying degrees; exogenous types generally have no obvious tongue movement disturbance and less spontaneous pain. Advanced tongue cancer may directly exceed the midline or invade the floor of the mouth, and may also infiltrate the lingual periosteum, bone plate or bone of the mandible. Later, it can extend to the base of the tongue ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
Inventor 朱进安
Owner SHANGHAI TENTH PEOPLES HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com