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Detection method for retinoic acid receptor alpha protein with nuclear localization signal

A technology for retinoic acid receptor and positioning signal, applied in the field of detection of retinoic acid receptor alpha protein

Active Publication Date: 2013-11-27
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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Problems solved by technology

And it has been reported in the literature that PML-RARα is transfected into early myeloid cells, which can eas

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  • Detection method for retinoic acid receptor alpha protein with nuclear localization signal
  • Detection method for retinoic acid receptor alpha protein with nuclear localization signal
  • Detection method for retinoic acid receptor alpha protein with nuclear localization signal

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Embodiment Construction

[0029] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as the conditions described in Cell Culture Technology (Third Edition, Gu Hongxi et al., Peking University Medical Press Co., Ltd.), or according to the manufacturer's instructions. recommended conditions.

[0030]In the following examples, the main reagents involved are: HL-60 and K562 cell lines were purchased from the Cell Resource Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; RPMI1640 medium for culturing HL-60 cells, fetal bovine serum (FBS ) were purchased from GIBCO Company of the United States; RPMI1640 medium and fetal bovine serum (FBS) for cultivating K562 cells were purchased from Hyclone Company of the United States; total RNA extraction kits, hi...

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Abstract

The invention relates to the biological field, specifically relates to detection techniques of proteins and particularly relates to a detection method for a retinoic acid receptor alpha protein with a nuclear localization signal. The detection method is a laser scanning confocal method and comprises the following steps of putting a dyed target cell into a two-tone fluorescence channel with the adequate wavelength of a laser scanning confocal microscope to capture fluorescence strength data of each part in the target cell, and analyzing the fluorescence strength data of each part in the target cell so as to judge whether the retinoic acid receptor alpha protein with the nuclear localization signal exists in the target cell. Besides, according to the detection method, multiple methods for detecting the NLS-RAR alpha (nuclear localization signal-retinoic acid receptor alpha) protein are integrated to lay the foundation for further research on the early diagnosis and recurrence monitoring of acute promyelocytic leukemia.

Description

technical field [0001] The invention relates to the field of biology, in particular to a protein detection technology, in particular to a detection method for a retinoic acid receptor alpha protein with a nuclear localization signal. Background technique [0002] Acute promyelocytic leukemia (APL) is a malignant clonal disease of hematopoietic stem cells. It is a special type of acute myeloid leukemia with rapid onset and high mortality. It is defined in the FAB classification system as M3 type. A large number of clinical studies have found that about 97% of APL have a characteristic t(15;17)(15q22;17q21) chromosomal translocation, forming a promyelocytic leukemia-retinoic acid receptor α fusion gene (PML-RARα). The PML-RARα fusion protein expressed by this gene is the molecular basis for the occurrence and development of APL. However, the PML-RARα fusion protein does not always function as a whole. Lane et al. [2] It was found that neutrophil elastase (Neutrophil Elasta...

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Application Information

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IPC IPC(8): G01N33/68G01N21/64
Inventor 刘北忠王慧钟梁朱新瑜马鹏鹏胡秀秀高远梅张曦
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
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