Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method used for separation and culture of poultry endothelial progenitor cells

A technology for the isolation and cultivation of endothelial progenitor cells, which is applied in the field of poultry tissue and cell culture engineering, and can solve problems such as the difficulty in obtaining poultry EPCs and the inapplicability of separating and culturing poultry EPCs

Inactive Publication Date: 2014-01-01
ZHEJIANG UNIV
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after many explorations, we found that it is difficult to obtain poultry EPCs by simply culturing adherent cells or non-adherent cells, indicating that the method of isolating and culturing mammalian EPCs is not suitable for isolating and culturing poultry EPCs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method used for separation and culture of poultry endothelial progenitor cells
  • Method used for separation and culture of poultry endothelial progenitor cells
  • Method used for separation and culture of poultry endothelial progenitor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 Materials and methods

[0019] 1.1 Experimental animals

[0020] 20-day-old healthy AA broiler chickens.

[0021] Handling of Cell Culture Plates

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method used for separation and culture of poultry endothelial progenitor cells (EPCs). The method comprises following steps: peripheral blood is collected and separated so as to obtain mononuclear cells; the mononuclear cells are cultured in a culture solution; and a mixture of adherent cells and non-adherent cells is used for culture. It is possible to obtain a large amount of EPCs by separation and culture of poultry peripheral blood using the method.

Description

technical field [0001] The invention relates to the field of poultry tissue and cell culture engineering, in particular to a method for isolating and culturing poultry endothelial progenitor cells. Background technique [0002] Endothelial progenitor cells (EPCs) are a type of precursor cells that can differentiate into vascular endothelial cells. They not only participate in embryonic angiogenesis, but also participate in postnatal angiogenesis and repair after vascular endothelial injury. When the vascular endothelium is damaged, EPCs in the systemic circulation migrate to specific sites for differentiation and proliferation under the action of various factors, and promote vascular repair. The vascular repair and directional migration of EPCs have shown broad application prospects in the field of cardiovascular disease treatment and gene targeting therapy, and are one of the hotspots in the field of biomedical research. [0003] At present, poultry has become an important...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 谭勋毕师诚
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com