Silkworm chrysalis active polypeptide after-sun repairing gel and preparation method thereof

A technology of silkworm chrysalis active polypeptide and after-sun repair, which is applied in the field of skin care products to achieve the effect of eliminating allergic factors

Active Publication Date: 2014-01-22
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Applying silkworm chrysalis to the cosmetics production industry has the following problems that need to be overcome and solved: 1. Silkworm chrysalis is a by-product after silk reeling, and generally has a relatively large odor. It is difficult to remove its peculiar smell when it is applied to cosmetics or other food preparations; 2. Silkworm chrysalis proteins are mostly globulins, and the solubility of globulins is poor. If it is directly applied to cosmetics preparations, it is not easy to be absorbed by the skin It must be converted into soluble substances that are easily absorbed by the skin; 3. Silkworm chrysalis protein is a material with high protein content. Since some people are allergic to high protein, if the protein components contained therein are directly applied to the cosmetics industry, it is also May cause allergies in some users

Method used

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  • Silkworm chrysalis active polypeptide after-sun repairing gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Preparation of silkworm chrysalis active polypeptide:

[0088] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and 92 ℃ inactivate the enzyme for 13 minutes;

[0089] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;

[0090] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfiltration membra...

Embodiment 2

[0119] (1) Preparation of silkworm chrysalis active polypeptide:

[0120](1) Prepare a silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymolysis temperature is 55°C, the pH value is 7.0, the enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;

[0121] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;

[0122] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed throug...

Embodiment 3

[0130] (1) Preparation of silkworm chrysalis active polypeptide:

[0131] (1) Prepare a silkworm chrysalis protein solution with a concentration of 15% in deionized water, add papain, the enzyme amount is 60000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;

[0132] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;

[0133] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra...

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Abstract

The invention relates to the technical field of skin care products, and in particular relates to silkworm chrysalis active polypeptide after-sun repairing gel. The silkworm chrysalis active polypeptide after-sun repairing gel comprises silkworm chrysalis active polypeptide, summer purslane extracts, pomegranate seed extracts, tremella polysaccharide, rose essential oil, nisin, gel matrix, wetting agents, penetrating agents, triethanolamine, edetic acid, corrosion remover and the balance of purified water or normal saline. The defects of silkworm chrysalis protein and silkworm chrysalis active polypeptide are overcome; the prepared after-sun repairing gel has no odor, is easily absorbed by the skin and can effectively reduce the damage of biomacromolecule caused by the sunshine, reduce the skin problems such as melanin deposition, sunburn, skin fine lines and the like caused by the sunshine, reduce the carcinogenic possibility caused by ultraviolet damage, and simultaneously prevent the dehydration of the skin, promote the growth, the differentiation and the proliferation of cells, and keep the elasticity and the quality of the skin; moreover, the gel has multiple after-sun repairing functions.

Description

technical field [0001] The invention relates to the technical field of skin care products, in particular to a silkworm chrysalis active polypeptide after-sun repairing gel, and also relates to a preparation method of silkworm chrysalis active polypeptide after-sun repairing gel. Background technique [0002] The ultraviolet rays radiated from the sun to the ground include UV-B with a wavelength of 290nm-320nm and UV-A with a wavelength of 320nm-400nm. The exposed skin is irradiated by ultraviolet rays, the surface of the skin will absorb UVB to generate free radicals, free radicals can damage DNA, collagen and other biological macromolecules, thereby eroding and destroying the skin surface cells, leading to skin aging, melanin deposition, sunburn and other various skin problem. UVA has stronger skin penetration than UVB, which can significantly increase the acute damage of UVA and increase the possibility of skin cancer. [0003] Free radicals are an important cause of s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/73A61K8/64A61Q19/00A61Q19/02C07K1/36C07K1/12C07K1/34
Inventor 刘玮杜芳岭陈相艳刘曼丽刘丽娜徐同成陶海腾
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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