Special method and device for rat intraperitoneal injection

Abstract

The invention provides a special method and device for rat intraperitoneal injection, wherein the method and equipment are low in rat stress and emergency response, safe, reliable and convenient to use. The method comprises the following steps that (1) according to the actual weight of a rat, a top cover in the corresponding size is selected; (2) an injector is used for pumping medicine inside, the rat is placed at a tunnel channel portal of the equipment and the rat can walk into the tunnel channel automatically; (3) after the rat enters the tunnel channel, the rat can walk or run forwards on a conveying mechanism by instinct, the tail of the rat is dragged and lifted up to prevent the rat from escaping forward, and the buttocks of the rat are made to turn upwards by about 15 degrees to 30 degrees; (4) the injector is used, a syringe needle is made to tilt upwards slightly and pierce into the position which is 1cm away from the right side of the lower middle abdomen, when an operator feels that the syringe needle drops obviously, the medicine is injected into the abdominal cavity quickly, and after the medicine is completely injected, the syringe needle is withdrawn quickly. The equipment is a channel so that the rat can move inside to a certain extent, therefore, struggling of the rat is avoided, the stress response is reduced and influences to the experiment result are reduced to the minimum.

Description

technical field [0001] The invention relates to a method and equipment specially used for intraperitoneal injection of rats. Background technique [0002] Rats are one of the most commonly used animals in animal experiments. They are often used in research in the fields of neuro-endocrine, behavior, nutrition and metabolic diseases, surgery, infectious diseases, pharmacology, genetics, oncology, etc. Rats have become a part of research, and as the world ages, more and more research is being done on aged rats. In experiments on rats, it is often necessary to inject intraperitoneal drugs, such as anesthetics and routine research drugs, into the rats. However, at present, intraperitoneal drug injection is mostly carried out under the condition that the rat is firmly grasped by hand or the activity of the rat is strongly restricted by using a rat immobilization device. These intraperitoneal drug injections under the condition of strongly restricting the activity of rats will f...

AI Technical Summary

Problems solved by technology

However, at present, intraperitoneal drug injection is mostly carried out in the situation where rats are grasped firmly by hand or rat immobilization device is used to forcefully restrict the rat's activities

These intraperitoneal drug injections under the condition of strongly restricting the activity of rats will force the rats to struggle violently, causing a strong stress response to the rats, and ultimately affecting the experimental results (especially neuro-endocrine and behavioral experiments), and Rats are often not grasped firmly (especially old rats), and drugs are easily injected into important blood vessels or organs in the abdominal cavity, resulting in the death of rats, and researchers are easily bitten by rats

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