A Plutella xylostella sex pheromone chemical communication disruptor
A technology of diamondback moth and pheromone, which is applied in the directions of chemicals, biocides, attractants for biological control, etc., can solve the problem of high control cost, and achieve the effects of reducing field population, convenient operation and low use cost.
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Embodiment 1
[0018] Observation on Courtship Behavior of Female Diamondback Moth
[0019] experiment method
[0020] The reagents are prepared, turpentine volatile matter: concentration is 100 μg / μl turpentine n-hexane solution; terpineol volatile matter: concentration is 100 μg / μl terpineol n-hexane solution; eucalyptol alcohol volatile matter: concentration is 100 μg / μl eucalyptus Leaf alcohol n-hexane solution.
[0021] Take 10 μl of n-hexane solution (H), turpentine volatiles (A), terpineol volatiles (B), and eucalyptol volatiles (C) respectively and load them into red natural silicone rubber stopper lures (diameter 1cm, length 1cm).
[0022] Select small green cabbage seedlings with a height of 80±5mm, and put them into a cotton-sealed test tube (the test tube is 2cm in diameter and 10cm long, and the cotton occupies a small amount of honey). From H40 to H40, the labels of test tubes containing turpentine volatiles are HA1 to HA40, the labels of test tubes containing terpineol vola...
Embodiment 2
[0028] wind tunnel device
[0029] The wind-pushing wind tunnel device consists of two parts: the wind source and the wind tunnel chamber. The wind source is provided by a manual gear fan at a constant speed, and the exterior is covered with black fine gauze; the wind tunnel chamber is composed of a transparent cylinder with a bottom diameter of 50cm and a length of 120cm, and is covered with fine iron sand mesh on both sides. The wind tunnel is divided into four areas: the downwind area S1 of the male moth release site, with a length of 30 cm; the upwind area S4 of the odor source, with a length of 30 cm; the area between S1 and S4 is divided into two areas on average, namely S2 and S2 near S1. The length of S3 close to S4 is 60cm.
[0030] A red light source is placed on the upper part of the wind tunnel, with adjustable brightness; the blowing device is on the right; the dark room where the wind tunnel is located has a temperature of 27±2°C and a humidity of 70-80%. Betwe...
Embodiment 3
[0057] field test
[0058] Lure preparation
[0059] The lure uses a red rubber stopper, absorbs 100 μl of the prepared three-component sex pheromone solution with a concentration of 0.1 μg / μl, and prepares a sex pheromone lure with a dose of 10 μg; the releaser of the interfering agent is a plastic centrifuge tube with a volume of 2 ml , Punch a hole at the cap of the bottle with a diameter of about 3mm, add 1.5ml paraffin oil solution of non-host volatiles to each releaser, the concentration is 1μg / μl.
[0060] Fix the sex pheromone red lure on the yellow plastic water basin with an iron wire; the plastic centrifuge tube with the non-host volatile matter crosses the iron wire upwards, 1 cm away from the red lure; the control is only adding n-hexane lure + releaser filled with paraffin oil solution in a volume of 1.5ml.
[0061] trap placement
[0062] Place the trap at a height of about 20cm from the surface of the vegetation, and add soapy water with a dilution of 3‰ (to...
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