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Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method

A technology of counting method and dilution device, which is applied in the field of microbiological inspection, to achieve the effect of improving work efficiency, ensuring success rate, and overcoming the hysteresis of counting results

Inactive Publication Date: 2015-01-07
沈佳特
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  • Abstract
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  • Application Information

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  • Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method
  • Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method

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[0070] 1. Machine installation:

[0071] Such as figure 1 , the airtight chamber is the main structural part of the device, which is an airtight chamber with an airtight door;

[0072] Nephelometer 6, vacuum sensor 7, pipeline cutting part 8, gas path sealing part 9, and liquid path opening part 10 are arranged inside the closed chamber 1;

[0073] The turbidimeter 6 is preferably arranged below the inside of the closed cavity 1, and its optical path passes through the bacteria solution tube 12;

[0074] The vacuum sensor 7 is preferably arranged on the inner side of the airtight cavity 1;

[0075] The pipeline cutting part 8 is preferably arranged above the inside of the closed chamber 1, above the liquid inlet pipe 11.3 of the dilution card 11, and moves downward to cut the liquid inlet pipe 11.3;

[0076]The gas path seal 9 is preferably arranged above the inside of the airtight chamber 1, on the side of the dilution card 11, and its lateral movement melts the gas path s...

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Abstract

The invention discloses an automatic continuous multi-stage dilution device for a bacterial suspension and a real-time counting method, and relates to the technical field of microbiological inspection. The device comprises a nephelometer, a vacuum degree sensor, a pipeline cutting piece, an air channel sealing piece and a liquid channel opening piece which are arranged in a closed cavity, wherein a negative pressure pump is connected with the closed cavity through an air channel switch; an air filter is connected with the closed cavity through an air inlet valve; a microcontroller is electrically connected with electrical appliance parts to form an integrated mechanical and electronic device; a liquid inlet pipe of a dilution card is inserted into a bacterial liquid pipe and is arranged in the closed cavity; the nephelometer is used for measuring the initial concentration of the bacterial suspension; the negative pressure pump performs vacuumizing to form negative pressure in the closed cavity and the dilution card, air is sucked, the bacterial suspension automatically enters a dilution pipe and is gradually diluted, a correction curve is established for real-time counting, a result is displayed on a touch screen, and the whole working flow is controlled to operate in a fully-automatic manner through the microcontroller. According to the device and the method, the bacterial suspension can be continuously and automatically diluted and counted in real time. The device and the method are intelligent, quick and convenient, and are applicable to microbiological inspection for food, drugs, hygiene series and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial inspection, and in particular relates to a bacterial suspension dilution device and a counting method; in particular, it relates to a continuous multi-stage automatic dilution device and a real-time counting method based on the turbidity of the bacterial suspension. Background technique [0002] In the field of food, medicine and medical and health microbiological testing technology, it is often necessary to dilute and count the bacterial suspension to obtain the required bacterial concentration, such as the "sterility test method" and "microbial limit test method" of the Chinese Pharmacopoeia 2010 edition And other laws and regulations clearly stipulate that the applicability inspection of the medium, the method verification of the inspection methods of each variety, and the positive control test are required, and it is required to use a certain number of specified strains to conduct related tes...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38G01N15/06
Inventor 沈佳特
Owner 沈佳特
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