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A kind of jute microsatellite DNA marker fingerprint and its application

A technology of DNA markers and fingerprints, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial measurement/inspection, etc., can solve the problems of high genetic similarity of local varieties, homonym of jute varieties, etc., and achieve detection time Short, high accuracy, good repeatability

Inactive Publication Date: 2016-06-08
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the country is concerned, due to the introduction and utilization of interregional varieties in the 20th century, the phenomenon of homonym of jute varieties is very serious, and the genetic similarity of local varieties is high. It is difficult to accurately and truly identify jute by traditional morphological and cytological identification methods. Variety differences, for which analysis and identification at the molecular level are required

Method used

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  • A kind of jute microsatellite DNA marker fingerprint and its application
  • A kind of jute microsatellite DNA marker fingerprint and its application
  • A kind of jute microsatellite DNA marker fingerprint and its application

Examples

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Embodiment 1

[0038] Example 1 Construction of jute microsatellite DNA marker fingerprints: according to the amplification situation of each primer (28 pairs of SSR primers) of each material in the above table 2, the DNA fingerprints of 58 jute varieties (lines) were drawn. In this DNA fingerprint, each jute variety (line) has its specific DNA fingerprint, which can distinguish these 58 varieties (lines) from each other (see figure 1 with Table 2). The names and sources of the 58 varieties (lines) are shown in Table 1.

Embodiment 2

[0039] Embodiment 2 uses jute DNA fingerprint collection of the present invention for the authenticity detection of certain jute germplasm resources: as the jute 179 kind in the test material is the contrast variety of the round fruit kind jute variety examination and approval, if there is a problem in the contrast kind, Will cause the entire region test to fail. It is uncertain whether the existing batch of seeds are real jute 179 seeds. Therefore, the DNA fingerprints of the batch of seeds are established according to the method of the present invention, and then compared with the standard spectra for identification. Using the characteristic primers CoSSR305 and CoSSR192 of "Jute 179" to test the authenticity of these jute varieties to be tested. A total of 192 samples were tested, among which 189 samples with "Jute 179" fingerprints (CoSSR305-120 and CoSSR192-380) were detected, accounting for 98.4% of the total number of samples tested. The identification result shows tha...

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Abstract

The invention relates to a corchorus microsatellite DNA marker fingerprint and applications thereof. The DNA fingerprint is constructed by DNA extraction, PCR amplification, identification of PCR products, and data analysis through taking combinations of 28 pairs of SSR primer alleles as a basis and 58 corchorus varieties / strains as test materials. The fingerprint can be used for distinguishing tested corchorus varieties / strains and can be used for identification or purity analysis of the corchorus varieties / strains. Compared with the traditional morphological and cytological identification test, the corchorus microsatellite DNA marker fingerprint has the advantages of being short in detection time, high in accuracy and good in repeatability.

Description

technical field [0001] The invention relates to a molecular marker technology, in particular to a fingerprint of jute microsatellite DNA marker and its application to identification or purity analysis of jute varieties / lines. Background technique [0002] Jute (Corchorus) is an important bast fiber crop second only to cotton in the world, mainly grown in India, Bangladesh and China. There are long fruit species (Corchorus olitorius L.) and round fruit species (Corchorus capsularis L.) cultivated for production, and the number of chromosomes is 2n=2X=14. Jute fiber is soft, strong, good hygroscopicity, fast water dispersal, good spinnability, and has a wide range of uses. [0003] High-quality jute varieties contribute significantly to jute yield and quality. my country's jute breeders have started the breeding research of jute since the 1940s, and successively released round fruit varieties such as Minma 369, Yueyuan No. 4, Meifeng No. 4, Minma No. 5, jute 179 and Bachang ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 张立武祁建民袁民航蔡蓉蓉
Owner FUJIAN AGRI & FORESTRY UNIV