Method for collecting mesenchymal stem cells through rat stress fracture model

Abstract

The invention provides a method for collecting mesenchymal stem cells by building a rat stress fracture model. The method comprises the steps that firstly, the rat stress fracture model fixed by an external fixing support is built, after modeling successes, fracture broken-end tissue is taken out, and the migrating cells are separated and cultured; the mechanical environment for fixing the fractured position is controlled, the mesenchymal stem cells are collected, and fracture healing is promoted. The method is easy to operate, scientific and reasonable, a shearing method is adopted to separate the migrating cells in the fracture broken-end tissues, the method is easy and convenient to implement, the injury to the cells is small, most of the cells in the fracture broken-end tissues can be separated out, operation time is short, the method can be applied to researching of the influence of the fracture healing early stress environment on the collection of the mesenchymal stem cells of the fracture broken end and further researching of indexes like differentiation of the mesenchymal stem cells, the early safe and effective rehabilitation plan of the fracture is guided and determined, and through the proper stress, the joint function is restored to be normal while more mesenchymal stem cells are collected.

Description

technical field [0001] The invention relates to a method for recruiting mesenchymal stem cells, in particular to a method for recruiting mesenchymal stem cells by establishing a rat stress fracture model. Background technique [0002] In the fields of tissue and organ injury repair, tissue engineering and disease treatment, despite the hot calls for embryonic stem cells, the most ideal cells are still autologous tissue cells. Therefore, how to obtain and utilize autologous tissue and autologous stem cells has always been a concern of researchers. [0003] At present, the source and application theory of stem cells can be roughly divided into three important directions. First, embryonic stem cells (ES); second, mesenchymal stem cells (MSC); Reprogramming transforms somatic cells to cultivate induced pluripotent stem cells (induced pluripotent stem cells, iPS). Due to the impact of ethical controversy, immune rejection, and experimental technical limitations, the current s...

AI Technical Summary

Problems solved by technology

However, the cellular mechanism of fretting to promote fracture healing is still not very clear. If the relationship between fretting and cellular molecular activities is elucidated, it will be possible to promote fracture healing by controlling the mechanical environment. The patient quantitatively controls the rehabilitation process so that the micro-movements caused by the rehabilitation process are always in a safe range that is conducive to fracture healing, so as to effectively avoid various fracture complications caused by blind rehabilitation and achieve the goal of fracture treatment

Images