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RT-PCR primers for detecting transcriptional level of HDAC1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population

A RT-PCR, transcription-level technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of application limitations, inability to accurately quantify mRNA, easy contamination, false positive rate, etc.

Inactive Publication Date: 2015-05-20
HUZHOU NO 1 PEOPLES HOSPITAL
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Problems solved by technology

However, in the application of traditional PCR technology, there are shortcomings such as inability to accurately quantify specific mRNA, and easy contamination during operation, resulting in a high false positive rate, so it cannot meet the needs of actual work and its application is limited.

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  • RT-PCR primers for detecting transcriptional level of HDAC1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population
  • RT-PCR primers for detecting transcriptional level of HDAC1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population
  • RT-PCR primers for detecting transcriptional level of HDAC1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population

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Embodiment Construction

[0075] Below in conjunction with embodiment the present invention is further described. However, it should be clear that the above description and the following examples herein are only for illustration, and do not constitute a limitation to the scope of the present invention.

[0076] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0077] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0078] 1. Primer Design and Synthesis

[0079] 1.1 Design and synthesis of HDAC1 gene primers

[0080]The HDAC1 gene standard sequence to be amplified is 1089-1264 bases in exon 10 and 11 of HDAC1 gene, and primers are designed using the full-length HDAC1 mRNA sequence as a template. The F sequence of the upstream primer of the standard PCR is: 5'- GCTCCACATCAGTCCTTCC -3'; the R sequence of the downstream primer is: 5'- GGTCGTCTTCGTCCTCATC -3'.

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Abstract

The invention relates to an RT-PCR kit and a method for evaluating the concurrent intestinal cancer susceptibility of a hyperglycemia population by using the kit, belonging to the technical field of real-time fluorescence quantitative reverse transcription polymerase chain reaction kit development and human pathology combination. RT-PCR primers for detecting the transcriptional level of HDAC1 mRNA in serum include a primer F: 5'-GCTCCACATCAGTCCTTCC-3' and a primer R: 5'-GGTCGTCTTCGTCCTCATC-3'. The invention establishes a method for detecting HDAC1 mRNA in peripheral vein serum by using the specific primers; and a quantitative PCR amplification technology is adopted in the method, so that the sensitivity for detecting HDAC1 mRNA is greatly improved, and enough information can be obtained in extremely few specimens.

Description

[0001] RT-PCR primers for detecting the transcript level of HDAC1 mRNA in serum, an RT-PCR kit for evaluating the susceptibility of hyperglycemia population complicated with colorectal cancer, and a method for evaluating the susceptibility of hyperglycemia population complicated with colorectal cancer. technical field [0002] The present invention relates to a primer, a kit and a method for evaluating the susceptibility of hyperglycemia population complicated with colorectal cancer; in particular, it relates to a RT-PCR primer for detecting HDAC1 mRNA transcription level in serum, which is used for evaluating hyperglycemia population complicated with colorectal cancer RT-PCR kit for susceptibility and method for assessing susceptibility to colorectal cancer in hyperglycemia population. It belongs to the technical field of the combination of real-time fluorescent quantitative reverse transcription polymerase chain reaction kit development and human pathology. Background tec...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q1/6844
Inventor 鲍鹰崔戈冯文明郭慧慧薛涛王翔
Owner HUZHOU NO 1 PEOPLES HOSPITAL
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