RT-PCR primers for detecting transcriptional level of NOX1 mRNA in serum as well as kit and method for evaluating concurrent intestinal cancer susceptibility of hyperglycemia population

A RT-PCR, transcription level technology, applied in DNA/RNA fragments, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems of inaccurate mRNA quantification, easy contamination, false positive rate, application limitations, etc.
CN104630348AInactive Publication Date: 2015-05-20HUZHOU NO 1 PEOPLES HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUZHOU NO 1 PEOPLES HOSPITAL
Publication Date
2015-05-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an RT-PCR kit and a method for evaluating the concurrent intestinal cancer susceptibility of a hyperglycemia population by using the kit, belonging to the technical field of real-time fluorescence quantitative reverse transcription polymerase chain reaction kit development and human pathology combination. RT-PCR primers for detecting the transcriptional level of NOX1 mRNA in serum include a primer F: 5'-ATAGCAGAAGCCGACAGG-3' and a primer R: 5'-CCAGTGAGACCAGCAATG-3'. The invention establishes a method for detecting NOX1 mRNA in peripheral vein serum by using the specific primers; and a quantitative PCR amplification technology is adopted in the method, so that the sensitivity for detecting NOX1 mRNA is greatly improved, and enough information can be obtained in extremely few specimens.
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Description

technical field

[0001] The present invention relates to a primer, a kit and a method for assessing the susceptibility of hyperglycemia crowd complicated with colorectal cancer; in particular, it relates to a RT-PCR primer for detecting NOX1 mRNA transcription level in serum, which is used for assessing hyperglycemia crowd complicated with colorectal cancer RT-PCR kit for susceptibility and method for assessing susceptibility to colorectal cancer in hyperglycemia population. It belongs to the technical field of the combination of real-time fluorescent quantitative reverse transcription polymerase chain reaction kit development and human pathology. Background technique

[0002] According to the statistics of the World Health Organization (WHO), more than 220 million people in the world suffer from diabetes, and 3.4 million people died of severe complications caused by high blood sugar in 2004. By 2030, the number of deaths due to diabetes will double again . Among the Chines...

Claims

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