A method for increasing the number of predatory organs of Arthrosporium oligosporum
A technology for Arthrosporium oligospores and predatory organs, which is applied in the field of applied microbiology, can solve the problems of affecting biocontrol effects, no public reports are found, and the effect of three-dimensional bacterial nets is unstable, and achieves the effect of improving nematicide ability.
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[0018] The present invention will be described in further detail below in conjunction with the accompanying drawings.
[0019] The medium used in this example is a conventional medium.
[0020] The implementation steps of the method for improving oligospore Arthrobotrys oligospora (Arthrobotrys oligospora) predator organ quantity of the present invention are as follows:
[0021] 1. Preparation of nematodes for experiments
[0022] Inoculate Caenorhabditis elegans on sterilized oat water medium, and culture at 28°C for 7 days or 8 days. Filter the nematodes with the Behmann funnel method, centrifuge the collected nematodes at 2000 rpm for 2 min, discard the supernatant, resuspend with sterile deionized water, and repeat three times. Finally, dilute the nematodes to 15 / ul.
[0023] 2. Preparation of spores
[0024] Put 50ml of CMA solid medium into a 250ml Erlenmeyer flask, sterilize at 121°C for 20 minutes, inoculate Arthrobusia oligospora blocks after the medium is cooled,...
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