Method for increasing amount of trapping organs of arthrobotrys oligospora

A technology of Arthrobium oligosporum and predatory organs, which is applied in the field of applied microbiology, can solve the problems of affecting the effect of biological control, unstable three-dimensional bacterial net effect, and no public reports have been found, and achieves the effect of improving the ability to kill nematodes.

Inactive Publication Date: 2015-05-27

YUNNAN UNIV

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AI-Extracted Technical Summary

Problems solved by technology

The production of three-dimensional bacterial nets by Arthropodium oligosporum is affected by many factors, and the effect of producing three-dimensional bacterial nets is unstable. The small number of traps often affects its biocontrol effect

[0004] The ...

Abstract

The invention relates to a method for increasing the amount of trapping organs of arthrobotrys oligospora serving as a nematode-trapping fungus, belonging to the field of applied microbiology. The final concentration of a spore suspension of the arthrobotrys oligospora is up to 20mM through adding sodium nitrate into the spore suspension of the arthrobotrys oligospora, so that the amount of the trapping organs of the arthrobotrys oligospora is increased, and the nematode killing capacity of the trapping organs of the arthrobotrys oligospora is improved. The method has the beneficial effects that a great number of trapping organs are generated by the arthrobotrys oligospora after NaNO3 is added in the spore suspension of the arthrobotrys oligospora, so that the nematode killing capacity is remarkably improved, and the death rate of nematodes is up to 85.88% when the nematodes are added for 12 hours; and when the nematodes are added for 24 hours, the amount of the trapping organs generated on a flat plate on which sodium nitrate is added is more than six times as much as the amount of the trapping organs generated on a flat plate on which no sodium nitrate is added, and the death rate of the nematodes is up to 99.80%.

Application Domain

FungiMicroorganism based processes +3

Technology Topic

Arthrobotrys oligosporaBiology +4

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Examples

Experimental program(1)

Example Embodiment

[0018] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0019] The medium used in this example is a conventional medium.

[0020] The implementation steps of the method for increasing the number of predatory organs of Arthrobotrys oligospora of the present invention are as follows:

[0021] 1. Preparation of experimental nematodes

[0022] Caenorhabditis elegans was inoculated on sterilized oat water medium and cultured at 28°C for 7 or 8 days. The nematodes were filtered by the Bayman funnel method, the collected nematodes were centrifuged at 2000 rpm for 2 min, the supernatant was discarded, and resuspended in sterile deionized water, repeated three times. Finally, the nematodes were diluted to 15/ul.

[0023] 2. Preparation of spores

[0024] A 250ml Erlenmeyer flask was filled with 50ml of CMA solid medium, sterilized at 121°C for 20 minutes, after the medium was cooled, inoculated with Arthrospora oligospora, and cultured at 28°C for 15 days to produce a large number of spores. The spores were washed with sterile water and collected by filtration through six layers of sterile lens tissue.

[0025] 3. Induction of predatory organs

[0026] Add sodium nitrate to the spore suspension of Arthropoda oligosporum to a final concentration of 20 mM, take 150 μl and spread it evenly on CMA medium, incubate at 28°C for 3 or 4 days, and add 96 100 or 100 nematodes were counted under the microscope at 12h and 24h, respectively, to count the number of traps and nematode mortality. Meanwhile, CMA medium without sodium nitrate was used as a control. Three replicates for each experiment. Nematode mortality was calculated as follows:

[0027] Nematode mortality = dead nematodes/added nematodes × 100%

[0028] 4. Experimental results

[0029] Table 1: Nematicidal effect of Arthrospora oligospora

[0030]

[0031] The results showed that adding NaNO to the spore suspension 3 Afterwards, the ability of Arthrosporium oligospora to kill nematodes was significantly improved, and the nematode mortality rate reached 85.88% at 12 hours (see Table 1). The improvement of nematicidal ability was mainly through the production of a large number of predatory organs (see the attached picture). When nematodes were added for 24 hours, the number of predatory organs produced on the plate with sodium nitrate was more than 5 times more than that without the addition of sodium nitrate.

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