Method for increasing amount of trapping organs of arthrobotrys oligospora

Abstract

The invention relates to a method for increasing the amount of trapping organs of arthrobotrys oligospora serving as a nematode-trapping fungus, belonging to the field of applied microbiology. The final concentration of a spore suspension of the arthrobotrys oligospora is up to 20mM through adding sodium nitrate into the spore suspension of the arthrobotrys oligospora, so that the amount of the trapping organs of the arthrobotrys oligospora is increased, and the nematode killing capacity of the trapping organs of the arthrobotrys oligospora is improved. The method has the beneficial effects that a great number of trapping organs are generated by the arthrobotrys oligospora after NaNO3 is added in the spore suspension of the arthrobotrys oligospora, so that the nematode killing capacity is remarkably improved, and the death rate of nematodes is up to 85.88% when the nematodes are added for 12 hours; and when the nematodes are added for 24 hours, the amount of the trapping organs generated on a flat plate on which sodium nitrate is added is more than six times as much as the amount of the trapping organs generated on a flat plate on which no sodium nitrate is added, and the death rate of the nematodes is up to 99.80%.

Description

Technical field: [0001] The invention relates to a method for increasing the number of prey organs of Arthrobotrys oligospora, which belongs to the field of applied microbiology. Background technique: [0002] Crop pathogenic nematodes are a serious hazard, and the direct economic losses caused by crop pathogenic nematodes are as high as 157 billion U.S. dollars every year worldwide. In my country, diseases caused by pathogenic nematodes have become the second largest type of diseases in agricultural production after fungal diseases, seriously threatening the safety of grain and economic crops in our country. For a long time, the control of nematodes has mainly relied on chemical pesticides, but due to the high toxicity and high residue of nematode chemical pesticides, it seriously affects the safety of agricultural products. In recent years, research on nematode microorganisms has provided people with another way—biological control. Nematode microorganisms refer to a grou...

AI Technical Summary

Problems solved by technology

The production of three-dimensional bacterial nets by Arthropodium oligosporum is affected by many factors, and the effect of producing three-dimensional bacterial nets is unstable. The small number of traps often affects its biocontrol effect

[0004] The method provided by the present invention to increase the number of prey organs of Arthrobotrys oligospora (Arthrobotrys oligospora) has not been found to be published in the same literature as the content of the present invention through literature search

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