Method for generating SSR molecular identification numbers of fruit trees based on SSR genotypes

A molecular ID, genotype technology, applied in the field of molecular markers of molecular biology, can solve problems such as unfavorable fingerprint information comparison

Inactive Publication Date: 2015-05-27
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Fingerprint technology is a conventional method to use DNA markers to construct a variety molecular identification system, but t

Method used

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  • Method for generating SSR molecular identification numbers of fruit trees based on SSR genotypes
  • Method for generating SSR molecular identification numbers of fruit trees based on SSR genotypes
  • Method for generating SSR molecular identification numbers of fruit trees based on SSR genotypes

Examples

Experimental program
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Embodiment 1

[0014] Embodiment 1 takes the pear tree as an example to further illustrate the present invention.

[0015] The varieties of pear trees participating in the test are as follows: 1-Baipisu, 2-Gaoping Rhubarb, 3-Hanyuan Dabai Pear, 4-Jimi, 5-Jinchuan Sydney, 6-Jinhuazao, 7-Jinzhou Dayali, 8- Binxian Laoyisheng, 9-Julu Xiaomuli, 10-Apple Pear, 11-Feihuang Pear, 12-Tianshengfu, 13-Botou Big Yali, 14-Wanrong Golden Pear, 15-Xianglong, 16 -Perfume, 17-Xiaoyifu, 18-Xinglong Pear, 19-Snowflake, 20-Yali, 21-Zhaoxian Daya Pear, 22-Zhengjia Pear, 23-Boshanchi Pear, 24-Chili Pear, 25 -Chonghua University, 26-Dangshan Crisp, 27-Librin, 28-Day Face Red, 29-I Don't Know, 30-Lv'anjiu, 31-Wai Yinhong Pear, 32-August Red, 33-Huahong Pear, 34-torches, 35-Longquan Crisp, 36-Lunan Huangpishui, 37-horseshoe yellow, 38-Cangxi Sydney, 39-pugua pear, 40-water hyacinth, 41-Weishanhong Sydney, 42-Wenshanhong Sydney, 43-Pocket, 44-Yunnan Yellow Sour Pear, 45-Dayexue, 46-Desheng Fragrant Pear, 47-Fuan J...

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Abstract

The invention provides a method for generating SSR molecular identification numbers of fruit trees based on SSR genotypes. The method comprises the following steps: screening SSR primers for fruit tree variety amplification and performing PCR amplification on the fruit tree varieties; after performing electrophoretic detection on the PCR amplification product, counting the genotypes of the fruit tree varieties at various SSR sites, arranging all the genotypes at various SSR sites in an ascending order before assignment, marking the non-deficient genotype as 00, and numbering the genotypes in order from 01 to 99, and then sequentially connecting the genotype numbers of each variety at various SSR sites in a certain SSR site order based on the standard, thereby forming the SSR molecular identification number of the fruit tree variety. The method takes the difference of the allele frequencies of various SSR sites due to such factors as selection, mutation and drift into full account.

Description

technical field [0001] The invention relates to the field of molecular markers in molecular biology, and specifically provides a method for generating a fruit tree SSR molecular ID number based on an SSR genotype. Background technique [0002] In recent years, fruit tree research and industry have achieved rapid development, the speed of breeding and promotion of new varieties has been accelerated, and a large number of excellent fruit tree varieties have been used in production. Establishing a set of simple, fast and reliable molecular identification system for fruit tree species, which is used for species identification in breeding and promotion links, is of great significance for improving breeding efficiency, accelerating the breeding process, maintaining the interests of breeders and producers, and ensuring the healthy development of the fruit tree industry. important and practical. [0003] SSR, also known as microsatellites and short tandem repeats (STR), is formed b...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895
Inventor 薛华柏李秀根张慧蓉杨健王龙王苏珂
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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