Method for calculating fractal dimension of microvessels of tissue

A technology of fractal dimension and calculation method, which is applied in the field of medical digital image analysis, can solve the problems that the calculation method of tissue microvascular fractal dimension has not yet been established, and achieve the effect of small error, simple calculation and effective reflection

Inactive Publication Date: 2015-05-27
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Application Information

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Problems solved by technology

Currently, there is no established method for calculating the fractal dimension of tissue microvessels

Method used

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  • Method for calculating fractal dimension of microvessels of tissue
  • Method for calculating fractal dimension of microvessels of tissue
  • Method for calculating fractal dimension of microvessels of tissue

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Embodiment 1

[0036] Example 1 Calculation method of fractal dimension of microvessels in glioma tissue

[0037] 1. Calculation of fractal dimension of microvessels in glioma tissue

[0038] according to figure 1 The shown flow chart calculates the fractal dimension of microvessels in glioma tissue, and the specific steps are as follows:

[0039] (1) Use a microtome to perform routine slices of the tissue to be inspected to obtain tissue slices;

[0040] (2) Perform anti-CD34 immunohistochemical staining on the tissue section obtained in step (1) to mark blood vessels (using DAB chromogenic method) to obtain a stained section, and then use a slice panoramic scanner to scan to obtain a digital section;

[0041] (3) Intercept 3-5 "hot spots" containing the most abundant microvessels from the digital slice obtained in step (2), the image pixels are 1920 * 1080, and named as image A;

[0042](4) Use the image analysis software Image J 1.46s on the image A obtained in step (3), extract the po...

Embodiment 2

[0050] Example 2 Application of Microvessel Fractal Dimension in Glioma Tissue

[0051] From 2006 to 2012, 83 patients with glioblastoma (56 cases from Chongqing Southwest Hospital, 27 cases from Beijing Tiantan Hospital) pathological tissue sections and clinical follow-up data were collected, statistically analyzed and mvFD values ​​were calculated and survival analysis was performed. The results are as follows: Figure 4 and Figure 5 As shown, the overall survival of patients with mvFD>1.06 glioblastoma ( Figure 4 A) and progression-free survival ( Figure 4 B) are significantly larger than those in patients with mvFD≤1.06. Among glioblastoma patients receiving chemotherapy, the progression-free survival of patients with mvFD>1.06 was significantly longer than that of patients with mvFD≤1.06, suggesting that mvFD>1.06 can be used as a standard for good chemotherapy response in glioblastoma patients ( Figure 5 A). Among patients who did not receive chemotherapy, there ...

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Abstract

The invention belongs to the field of medical digital image analysis and particularly relates to a method for calculating fractal dimension of microvessels of tissue. The method comprises the following steps: carrying out conventional slicing on the tissue to be tested, so as to obtain a tissue slice; carrying out immunohistochemical staining blood vessel marking on the tissue slice so as to obtain a stained slice, and then, photographing a two-dimensional structured image of the stained slice by adopting image acquisition equipment, so as to obtain a digital slice; extracting a microvessel-rich hot-spot area from the digital slice, and naming the microvessel-rich hot-spot area as an image A; extracting a stained positive target area from the image A based on a threshold method in an HSB (Hue-Saturation-Brightness) color space so as to obtain an image A1, and then, converting the image A1 into a binary system, so as to obtain an image B; carrying out image skeletonizing on the image B, so as to obtain an image C; calculating the fractal dimension mvFD of the image C by adopting a box-counting method. The method is simple in operation, simple and convenient in calculation and relatively small in error, and the obtained fractal dimension can be used for completely and effectively reflecting the form complexity and number of the microvessels of the tissue to be tested.

Description

technical field [0001] The invention belongs to the field of medical digital image analysis, in particular to a method for calculating the fractal dimension of tissue microvessels. Background technique [0002] Blood vessels are the conduits through which living things transport blood. Both normal and diseased tissues of the human body contain blood vessels, especially some diseased (such as malignant tumor) tissues contain abundant blood vessels and form a vascular network system. Pathological tissue slices are the main carrier for pathological diagnosis, in which the morphology and quantity of tissue blood vessels can be used as the basis for auxiliary diagnosis of certain diseases (such as malignant tumors). Current methods for assessing tissue vascular characteristics include vascular morphology description, vascular density calculation, and immunohistochemical staining of vascular-specific molecular markers. However, there is still a lack of indicators for quantitativ...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): G06F19/00G06T7/00
Inventor陈聪平轶芳时雨卞修武孔祥复
OwnerTHE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA