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Point-of-care assay for the detection of enteroviruses using lateral flow immunochromatographic technology

An enterovirus, lateral flow technology, applied in measurement devices, analytical materials, biomaterial analysis, etc., can solve problems such as misclassification or misdiagnosis

Inactive Publication Date: 2015-07-15
INNOMART PTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This diagnostic approach is highly subjective and prone to misclassification or misdiagnosis

Method used

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  • Point-of-care assay for the detection of enteroviruses using lateral flow immunochromatographic technology
  • Point-of-care assay for the detection of enteroviruses using lateral flow immunochromatographic technology
  • Point-of-care assay for the detection of enteroviruses using lateral flow immunochromatographic technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0112] experiment setup

[0113] 1. Buffer Preparation

[0114] Sample Pad Buffer

[0115] 10 mM phosphate buffer, 1% v / v isopropanol and 0.04% Tween were added together to form a mixture. The pH of the mixture was adjusted to 8.0 to form the sample pad buffer. Store the sample pad buffer at 4 °C.

[0116] conjugation buffer

[0117] The conjugation buffer was prepared using 0.1 M MES (2-(N-morpholino)ethanesulfonic acid) buffer adjusted to pH 4.7 to 5.5. Store the conjugation buffer at 4 °C.

[0118] running buffer

[0119] 1 mM sodium borate, 1% isopropanol and 0.04% Tween were added together to form a mixture. The pH of the mixture was adjusted to 8.0 to form a running buffer. Store the running buffer at 4 °C.

[0120] 2. The structure of the lateral flow bar

[0121] figure 1 The structure of the lateral flow test strip of the present disclosure is shown. Lateral flow test strip assembly was performed using the BioDot Batch Laminator System (BioDot, CA, USA) ...

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Abstract

The present disclosure relates to a point-of-care lateral flow immunochromatographic assay for direct detection of enteroviruses. In particular, the present disclosure relates to a point-of-care lateral flow immunochromatographic assay for detection of the etiologic agents of Hand, Foot and Mouth Disease (HFMD), using antibodies specific for enteroviruses.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to and is the subject of Singapore Patent Application No. 201206800-3 entitled "Point-Of-Care Assay For The Detection Of Enteroviruses Using Lateral Flow Immunochromatographic Technology" filed on September 12, 2012 Transformation, which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates to point-of-care lateral flow immunographic assays for the detection of viruses. In particular, the present disclosure relates to in situ lateral flow immunochromatographic assays for the detection of enteroviruses. Background technique [0004] Hand, Foot and Mouth Disease (HFMD) is one of the most common infectious diseases in the Asia-Pacific region, causing many deaths in the past few decades. The disease is mainly spread through contact with bodily fluids such as saliva. Most enteroviruses are causative agents of HFMD. The prognosis for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N30/90
CPCG01N33/56983G01N33/558G01N2333/085G01N33/54386G01N33/54388
Inventor 李佳俊陈恩李
Owner INNOMART PTE
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