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Humanized mouse

A humanized, mouse technology, applied in the field of humanized mice

Active Publication Date: 2015-09-16
杭州正因生物技术有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the disadvantage that the mouse CD47 expressed in human cells cannot smoothly transmit extracellular signals into the cells in the existing technology of preparing humanized mice. The present invention provides a human This mouse can not only enable mouse phagocytes to recognize human cells as their own cells and no longer phagocytize human cells, but also enable human cells to obtain normal extracellular information in mice, and promote their Normal growth, development and function in mice, thus improving the application value of humanized mice

Method used

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Embodiment Construction

[0016] The present invention will be further described below with reference to the accompanying drawings and examples (the following sequence ID 1 is the fusion CD47 sequence of the present invention).

[0017] 1. Construction of human-mouse CD47 fusion gene

[0018]First, the 1st to 142nd amino acid residues of mouse CD47 (such as GENBANK accession number: AB012693) are fused with the 145th to 305th amino acid residues of human CD47 (such as GENBANK accession number: BT006907), and the corresponding nucleoside The acid sequence is sequence ID 1. According to the designed sequence, add three protective nucleotides and XbaI restriction endonuclease recognition sequence and Kozak sequence GCCACC before the 5' end ATG, and add BglII restriction endonuclease recognition sequence after the stop codon and three protected nucleotides. The sequence DNA was entrusted to a biotechnology company to synthesize.

[0019] The above-synthesized DNA was digested with BglII and XbaI, and th...

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Abstract

The invention discloses a humanized mouse. A mouse-human fusion CD47 gene is expressed on humanized cells of the humanized mouse and is shown in SEQ ID NO.1; an extracellular portion of the gene is a mouse CD47 sequence, and a transmembrane portion and an intracellular portion are human CD47 sequence; or the extracellular portion and the transmembrane portion are mouse CD47 amino acid sequences, and the intracellular portion is a human CD47 amino acid sequence. The humanized mouse has the humanized cells of the mouse-human fusion CD47 gene, extracellular information in an in-vivo environment of the mouse can be transmitted to the humanized cells and can be perceived by the humanized cells and the humanized cells have corresponding responses under the condition that the humanized cells are not swallowed by phagocytic cells, and the humanized cells in the humanized mouse grow and develop normally and play functions.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and relates to a humanized mouse, in particular to a humanized mouse that expresses a human-mouse fusion CD47 gene in human-derived cells to promote the normal growth and development of human-derived cells in the mouse body and exert functions. mouse. Background technique [0002] The translation of research findings in human immunology and immunopathology into clinical therapies is often limited by the inability to directly conduct in vivo experiments in humans. Although mouse models of human diseases can provide a lot of useful information, due to the significant differences between human physiology and animal physiology, experimental results obtained using animal models sometimes cannot be applied to humans, and non-human primate studies can fill in the gaps. There are gaps in the gap between species that are too distantly related, but their use is limited by their high cost, lack of inbred...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027
Inventor 王彦刈
Owner 杭州正因生物技术有限公司
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