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A flow chamber for biological fluid experiments
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A technology of biological fluid and flow chamber, applied in the field of biomedical experimental devices, can solve the problems of low efficiency, lack of versatility, high test cost, etc., and achieve the effects of improved effect, simple structure and good experimental effect
Active Publication Date: 2017-12-01
CHONGQING UNIV +1
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In the experimental research of biomedical engineering, for various cells such as vascular endothelial cells and blood cells, under the action of fluid force, the experimental research on cell proliferation and adhesion has been carried out in depth; however, due to the particularity of vascular structure , and the focus of the research on fluid mechanics and blood vessels is different, the above-mentioned experiments all need to design personalized experimental instruments; and because of the strong personalization of this research, there are almost no general-purpose vascular bionic cell fluid experimental instruments on the market; most of the research Mechanisms are all experimental instruments designed by themselves according to their own experimental needs; the flow chamber for biological fluid experiments is the core accessory of the experimental platform in fluid experimental instruments; it directly determines whether the experiment is successful or not, the experimental design, and the rationality of parameters. It is judged that the direct acquisition of experimental cell images and experimental data has played a crucial role; moreover, for the different sizes of the experimental platform, the requirements for the liquid circulation and flow rate required for the hydrodynamic experiment, the required flow chamber flow The size of the chamber is different, so it is necessary to specially design a flow chamber for biological fluid experiments to meet the experimental requirements; and it is matched with a specially designed cell fluid experiment instrument for cell fluid mechanics experiments, which makes the cost of each experiment higher. The specially designed flow chamber and supporting instruments do not have universality, and the use efficiency is low; in short, the flow chamber for biological fluid experiments in the prior art has problems such as poor versatility and low use efficiency.
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[0023] Such as Figure 1 to Figure 6As shown, a flow chamber for biological fluid experiments, the flow chamber includes an upper piece 13 and a lower piece 1 with a rectangular horizontal plane, and sequentially set from the upper piece 13 to the lower piece 1. Upper sheet groove 14 sealing gasket 18, cell slide 20, flow chamber sealing gasket 17 and glass sealing sheet 6, its upper sheet 13 and lower sheet 1 are connected by bolts; said upper sheet 13 is provided with upper sheet opening 15, upper sheet Sheet groove 14 and upper sheet bolt hole 7, its upper sheet opening 15 is through and arranged in the middle position of upper sheet 13, upper sheet groove 14 is arranged a week along the outer side of upper sheet opening 15 edge, notch is downward, and its edge length and width dimension and upper sheet The size of the sealing gasket 18 of the sheet groove 14 is matched, and the bolt hole 7 of the upper sheet is arranged on the outside of the upper sheet groove 14; the seal...
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Abstract
The invention relates to a flowing chamber for a biofluid experiment. The technical scheme of the lowing chamber for that biofluid experiment is that the flowing chamber comprises an upper piece, a lower piece, an upper piece groove sealing washer, a cellular glass slide, a flowing chamber sealing gasket and a glass sealing piece, wherein the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are arranged sequentially from the upper piece to the lower piece, horizontal planes of the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are rectangular, the length and width dimensions of the upper piece groove sealing washer, the cellular glass slide, the flowing chamber sealing gasket and the glass sealing piece are same, and the upper piece and the lower piece are connected through bolts; the flowing chamber for the biofluid experiment has the advantages that the structure is simple, and the use is convenient; at the same time, the practicability is high, the size of the flowing chamber can be changed by simply adjusting the thickness of the flowing chamber sealing gasket, accordingly, a flexible effect is realized, and various experiment requirements are met; particularly, several stages of buffering flow balancing structures are designed at an inlet and an outlet of the flowing chamber, and in and out of fluid both pass through all stages of buffering flow balancing so that the experiment data are more accurate, and the experiment effect is better.
Description
technical field [0001] The invention relates to a biomedical experiment device, in particular to a flow chamber for a biofluid experiment. Background technique [0002] A flow chamber for biological fluid experiments is an experimental component with a flow chamber that can be observed through a microscope. In the experimental research of biomedical engineering, for various cells such as vascular endothelial cells and blood cells, under the action of fluid force, the experimental research on cell proliferation and adhesion has been carried out in depth; however, due to the particularity of vascular structure , and the focus of the research on fluid mechanics and blood vessels is different, the above-mentioned experiments all need to design personalized experimental instruments; and because of the strong personalization of this research, there are almost no general-purpose vascular bionic cell fluid experimental instruments on the market; most of the research Mechanisms are ...
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