Rapid Propagation Method of Embryogenic Callus of Qiufeng
An embryogenic callus and rapid technology, which is applied in the field of plant propagation, can solve the problems that seed propagation cannot meet the market demand, and seeds are only available once a year, so as to enhance the ability to resist diseases and insect pests, improve the quality of seedlings, and increase the multiplication coefficient. Effect
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Embodiment 1
[0034] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:
[0035] A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 15 minutes, then soak them in 0.1% mercuric chloride solution added with 2 drops of 100 mg / L Tween 20 for 8 minutes, and then sterilize them with sterile water. Rinse 3 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;
[0036] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 22°C under light conditions to obtain sterile test-tube plantlets, wherein,
[0037] The first medium comprises MS medium, 0.1mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,
[0038] The light conditions are: light intensity 1500lux, light time 8h / day;
[0039] C. Place the cotyledons of the sterile ...
Embodiment 2
[0050] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:
[0051]A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 18 minutes, then soak and disinfect them with 0.1% mercuric chloride solution added with 2 drops of 100 mg / L Tween 20 for 9 minutes. Rinse 4 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;
[0052] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 24°C under light conditions to obtain sterile test-tube plantlets, wherein,
[0053] The first medium comprises MS medium, 0.3mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,
[0054] The light conditions are: light intensity 1500lux, light time 9h / day;
[0055] C. Place the cotyledons of the sterile test-tube plantlets obtained ...
Embodiment 3
[0066] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:
[0067] A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 20 minutes, and then soak them in 0.1% mercuric chloride solution added with 3 drops of 100 mg / L Tween 20 for 10 minutes. Rinse 5 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;
[0068] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 26°C under light conditions to obtain sterile test-tube plantlets, wherein,
[0069] The first medium comprises MS medium, 0.5mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,
[0070] The light conditions are, light intensity 1500lux, light time 10h / day;
[0071] C. Place the cotyledons of the sterile test-tube plantlets obtained in step B...
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