Rapid Propagation Method of Embryogenic Callus of Qiufeng

An embryogenic callus and rapid technology, which is applied in the field of plant propagation, can solve the problems that seed propagation cannot meet the market demand, and seeds are only available once a year, so as to enhance the ability to resist diseases and insect pests, improve the quality of seedlings, and increase the multiplication coefficient. Effect

Inactive Publication Date: 2017-04-26
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, autumn maple is propagated by seeds, but the seeds are only once a year, and the germination rate can be improved by harvesting and sowing. Seed propagation can no longer meet the needs of the market.
Tissue culture is a rapid plant propagation technology, but there is no report on the rapid propagation of autumn maple seedlings by tissue culture technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:

[0035] A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 15 minutes, then soak them in 0.1% mercuric chloride solution added with 2 drops of 100 mg / L Tween 20 for 8 minutes, and then sterilize them with sterile water. Rinse 3 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;

[0036] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 22°C under light conditions to obtain sterile test-tube plantlets, wherein,

[0037] The first medium comprises MS medium, 0.1mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,

[0038] The light conditions are: light intensity 1500lux, light time 8h / day;

[0039] C. Place the cotyledons of the sterile ...

Embodiment 2

[0050] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:

[0051]A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 18 minutes, then soak and disinfect them with 0.1% mercuric chloride solution added with 2 drops of 100 mg / L Tween 20 for 9 minutes. Rinse 4 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;

[0052] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 24°C under light conditions to obtain sterile test-tube plantlets, wherein,

[0053] The first medium comprises MS medium, 0.3mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,

[0054] The light conditions are: light intensity 1500lux, light time 9h / day;

[0055] C. Place the cotyledons of the sterile test-tube plantlets obtained ...

Embodiment 3

[0066] A method for rapid propagation of autumn maple embryogenic callus seedlings, comprising the following steps:

[0067] A. After soaking the autumn maple seeds with 2% aqueous solution of detergent for 5 minutes, rinse them with linear tap water for 20 minutes, and then soak them in 0.1% mercuric chloride solution added with 3 drops of 100 mg / L Tween 20 for 10 minutes. Rinse 5 times, and finally remove the surface moisture of the autumn maple seeds with sterilized filter paper to obtain sterilized explants;

[0068] B. Inoculate the sterilized explants obtained in step A into the first culture medium, and cultivate them for 35 days at 26°C under light conditions to obtain sterile test-tube plantlets, wherein,

[0069] The first medium comprises MS medium, 0.5mg / L gibberellin, 30mg / L sucrose and 5mg / L agar,

[0070] The light conditions are, light intensity 1500lux, light time 10h / day;

[0071] C. Place the cotyledons of the sterile test-tube plantlets obtained in step B...

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PUM

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Abstract

The invention provides a method for rapid propagation of bischofia javanica based on embryogenic callus seedling development. The method comprises the steps of explants selection and sterilization, sterile test-tube plantlet acquisition, embryoid induction culture, embryoid strong seedling culture, acclimatization and transplantation and the like. By the adoption of the method, industrialized bischofia javanica seedling culture is achieved effectively. The method has the advantages that culture time is short, propagation coefficient is high, survival rate is high, and plants are robust and can meet the requirements of the market for bischofia javanica seedlings.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for rapid propagation of autumn maple embryogenic callus into seedlings. Background technique [0002] Autumn maple, also known as Chongyang wood, is a tall tree with a simple and dignified appearance, a huge crown, and dense branches and leaves. It is a good tree species for roads and gardens. In addition, Qiufeng has a well-developed root system and strong wind resistance, so it can be built as a shelter forest. The whole body of autumn maple is a treasure. The wood texture is straight, hard and durable. It can be used for building furniture and bridges; the pulp can be used to make wine; The leaves can be used for esophageal cancer, gastric cancer, infectious hepatitis, infantile malnutrition, pneumonia, pharyngitis, sores, etc. [0003] At present, the autumn maple is propagated by seeds, but the seeds are only once a year, and the germination...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G9/10
Inventor 王晓峰李刚韦坤华梁莹缪剑华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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