Detecting β in Animal Tissues Using Transcriptional Markers 2 Agonist approach

Abstract

The invention discloses the use of transcription markers to detect β in animal tissues 2 Agonist approach. The method provided by the present invention comprises: using fluorescent quantitative PCR to respectively amplify ADRB2, PRKACB, ADCY3, ATP1A3, ATP2A3, PTH, MYLK, TNF-α, IL1B genes and internal reference genes in the isolated animal tissues and / or organs According to the difference between each gene and the Ct value in the internal reference gene system and the corresponding formula, y1 and y2 are obtained respectively; according to the following method, determine whether the animal tissue and / or organ contains or is candidate to contain β 2 Receptor agonist: if y1≥y2, the animal tissue to be tested does not contain or candidate does not contain β 2 Receptor agonist, such as y1<y2, the animal tissue to be tested contains or is candidate to contain β 2 receptor agonist. The present invention can effectively solve β 2 The monitoring problem of receptor agonists, effectively realize the detection of β 2 Agonist monitoring.

Description

technical field [0001] The invention relates to the use of transcription markers to detect β in animal tissues in the field of biotechnology 2 Agonist approach. Background technique [0002] Beta agonists include nonselective beta agonists such as isoproterenol and selective cardiac beta 1 Agonists such as dobutamine, selective beta 2 Receptor agonists such as albuterol, tert-butyl, and other drugs. beta 2 Receptor agonist which excites the β on the surface of airway smooth muscle and mast cell membrane 2 Receptors, relax airway smooth muscle, reduce mast cell and basophil degranulation and the release of their mediators, reduce microvascular permeability, increase the swing of airway epithelial cilia, etc. to relieve asthma symptoms. beta 2 Agonists include: cimaterol, penbuterol, tobuterol, sibuterol, bromobuterol, mapbuterol, terbutaline, chlorprenaline, ractopamine, gram Renbuterol, albuterol, bambuterol, ritodrine, clenpro, zilpaterol, mapenterol, etc. [0003] ...

AI Technical Summary

Problems solved by technology

[0004] Although the current Chinese standards and related laws and regulations have explicitly prohibited the addition of β to animal feed and drinking water 2 Receptor agonists, but they are still repeatedly banned, and the current mainstream drug residue analysis methods such as instrument analysis, immunoassay, sensor and chip analysis are all based on the detection of known drugs, and new drugs, as a loophole in monitoring, continue to threaten consumers. Personal safety

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