Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Detection of streptococcus pneumoniae

A Streptococcus pneumoniae and probe technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problem that there is no specific detection of Streptococcus genus

Active Publication Date: 2016-06-15
GENOMICA SAU
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no specific probes are provided for the specific detection of different species of Streptococcus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection of streptococcus pneumoniae
  • Detection of streptococcus pneumoniae
  • Detection of streptococcus pneumoniae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Samples of blood culture positive for S. pneumoniae were obtained and DNA was extracted therefrom by means of the NucliSENSeasy MAG kit from BioMerieux. The initial volume of blood culture used was 1 ml and the final volume after elution was 80 μl.

[0091] Next, take an elution volume of 5 μl and amplify it with a mixture of the following reagents:

[0092]

[0093]

[0094] Amplification products were denatured by incubation at 95°C for 10 minutes. Afterwards, in the presence of a standard hybridization solution, 5 μl of the denatured PCR product was mixed with a protein containing SEQID immobilized at a known position. and SEQID Microarray incubation of probes.

[0095] After a series of standard washing and blocking steps, images were taken to obtain figure 1 A visualization of the final result is shown in .

Embodiment 2

[0097] Samples of blood cultures positive for S. light were obtained and the same procedure described in Example 1 was performed for S. pneumoniae. exist figure 2 A visualization of the final result is shown in .

Embodiment 3

[0099] Samples of blood cultures positive for oral Streptococcus were obtained and the same procedure described in Example 1 was performed for Streptococcus pneumoniae.

[0100] exist image 3 A visualization of the final result is shown in .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention is based on a detection method of Streptococcus pneumoniae in a sample, which allows specifically distinguish Streptococcus pneumoniae from other species of the Streptococcus genus. Further, the present invention relates to (i) a kit which comprises a microarray wherein probes of the present invention are provided; (ii) the set of probes itself; (iii) a microarray comprising the set of probes; and (iv) use of the method, kit, set of probes, and microarray of above for detection of Streptococcus pneumoniae in a sample.

Description

Background of the invention [0001] Streptococcus pneumoniae is the causative agent of sepsis and it would be convenient to be able to specifically detect its presence in blood. Streptococcus pneumoniae is also a major cause of community-acquired pneumonia. It is very important to be able to distinguish S. pneumoniae from other members of the Streptococcus group, thereby determining whether any Streptococci present in the sample actually correspond to the pathogenic S. Other harmless streptococcal species that cause disease. [0002] Many attempts have been made to detect S. pneumoniae and differentiate it from other members of the Streptococcus group (Wessels et al., J. Clin. Microbiol. Apr. 2012, Vol. 50, No. 4, 1171-1177). These include culture tests and routine biochemical and phenotypic tests such as Gram-stained morphology, optoxin sensitivity, colony morphology, and alpha-hemolysis on sheep blood agar. Additional techniques used include: MALDI-TOF (MS matrix-assisted ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
CPCC12Q1/6837C12Q1/689
Inventor 安娜·伊莎贝尔·莫拉加金塔尼利亚玛丽亚·路易莎·比利亚埃尔莫萨贾因
Owner GENOMICA SAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com