Antagonistic bacteria of radix pseudostellariae root rot and application thereof

A technology of antagonistic bacteria and heterophylla, applied in the field of bacterial strains and antagonistic bacteria, can solve the problems of farmland ecosystem function degradation, aggravate environmental pollution, increase production cost input, etc., and achieve good rhizosphere colonization effect and strong cellulose degradation ability, the effect of strong phosphorus-dissolving ability

Active Publication Date: 2016-07-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

What's more serious is that when the causes and mechanism of continuous cropping obstacles are not yet clear, most farmers try to maintain production through measures such as increasing fertilizers, spreading lime, and abusing pesticides. A series of problems such as cost input, aggravation of environmental pollution, and degradation of farmland ecosystem functions have caused the cultivation and production of heterophylla pseudostellariae to fall into a vicious circle

Method used

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  • Antagonistic bacteria of radix pseudostellariae root rot and application thereof
  • Antagonistic bacteria of radix pseudostellariae root rot and application thereof
  • Antagonistic bacteria of radix pseudostellariae root rot and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening and identification of antagonistic bacteria Burkholderia ambifaria LK-P4

[0023] 1. Screening of antagonistic bacteria

[0024] The antagonistic bacteria Burkholderia ambifaria LK-P4 of the present invention is screened from the rhizosphere soil of Pseudostellaria heterophylla.

[0025] 1.1LB medium preparation

[0026] Weigh 5g LB dry powder (Haibo Biotechnology Co., Ltd., China) and 3g agar powder (BIOSHARP, Japan), heat and dissolve with deionized water and dilute to 200mL, autoclave at 115°C for 20min, and wait until the temperature of the medium drops to 55°C Add natamycin (fungal antibiotic) when the temperature is around ℃ (touchable by hand) to prevent the antibiotic from becoming ineffective due to high temperature, shake well and pour the plate quickly.

[0027] 1.2 Rhizosphere soil bacterial culture

[0028] Weigh 10g of heterophylla rhizosphere soil, dissolve it in 90mL of sterile water, shake it well, and get 10 -1 Diluent. Take 10...

Embodiment 2

[0037] Antibacterial effect detection of embodiment 2 antagonistic bacteria

[0038] Use PDA medium, inoculate activated Burkholderia LK-P4 at a distance of 2.5 cm from the center of the petri dish, place it in a constant temperature incubator at 28°C for 48 hours, and inoculate each Pathogen-like fungi were placed in a constant temperature incubator at 28°C in the dark for several days, and the formation of the inhibition zone was observed in real time. After 4 days of culture, it was found that Burkholderia ambifaria LK-P4 could effectively antagonize the Fusarium oxysporum specialized in heterophylla, Fusarium moniliforme and Fusarium moniliforme, and inhibit the mycelium growth (such as image 3 shown).

Embodiment 3

[0039] Example 3 Optimization of the optimal medium for Burkholderia ambifaria LK-P4

[0040] Prepare the following medium to optimize the optimal growth medium for Burkholderia LK-P4. The medium is as follows: 1) full LB solution; 2) 1 / 4LB solution; 3) 1 / 4LB+1 / 20MS; 4) 1 / 4LB+1 / 40MS; 5) 1 / 4LB+1 / 80MS; 6) 1 / 4LB+1 / 20MS+1wt.% brown sugar; 7) 1 / 4LB+1 / 40MS+1wt.% brown sugar; 8) 1 / 4LB+1 / 80MS+1wt.% brown sugar.

[0041] Among them, the formula of MS medium (no iron salts) is: 1) macroelements: 1900mg / LKNO 3 , 1650mg / LNH 4 NO 3 , 370mg / LMgSO 4 ·7H 2 O. 170mg / LKH 2 PO 4 , 440mg / LCaCl 2 2H 2 O; 2) Trace elements: 22.3mg / LMnSO 4 4H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 6.2mg / LH 3 BO 3 , 0.83mg / LKI, 0.25mg / LNa 2 MoO 4 ·7H 2 O, 0.025mg / LCuSO 4 ·5H 2 O, 0.025mg / LCoCl, 2.6mg / LH 2 O; 3) Organic matter: 2.0mg / L glycine, 0.5mg / L pyridoxine hydrochloride, 0.1mg / L ammonium sulfate hydrochloride, 0.5mg / L niacin, 100mg / L creatine; LB medium formula is: pancreas Peptone 10g / L, yeast extra...

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PUM

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Abstract

The invention aims to provide antagonistic bacteria of radix pseudostellariae root rot and application thereof. The antagonistic bacteria is identified as burkholderia which is named as Burkholderia ambifaria LK-P4 with a preservation number of CGMCC NO.12120. The bacteria are screened from rhizosphere soil of the radix pseudostellariae, have strong antagonistic effect on special-form pathogenic bacteria such as fusarium oxysporum and fusarium moniliforme which are separated from the diseased root part of the radix pseudostellariae, are good in rhizosphere colonization capacity and are free from pathogenicity on other after-culture crops. Meanwhile, an optical culture medium for the growth of the antagonistic bacteria is provided, wherein the optimal formula comprises 1/4 LB, 1/40 MS (not containing ferric salt), and 1wt% of brown sugar, so that a fermented liquid has obvious antagonistic effect. In order to overcome or relieve replanting disease of medicinal plant radix pseudostellariae, the invention provides antagonistic bacteria culture and a preparation, which are good in bio-control effect, free from pollution, safe and ecological, and wide in development application prospect.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium capable of antagonizing Pseudostellaria root rot, which can be used to overcome or alleviate the problem of continuous cropping Pseudostellaria soil-borne diseases, and belongs to the technical field of microorganisms and biological control. Background technique [0002] The problem of crop continuous cropping obstacles has existed for a long time. Studies have shown that there are varying degrees of continuous cropping obstacles in soybeans, cucumbers, peppers, tomatoes, peanuts, potatoes, watermelons, and strawberries. In recent years, reports on continuous cropping obstacles of medicinal plants such as heterophylla, ginseng, rehmannia glutinosa, lily, and salvia miltiorrhiza have gradually increased. [0003] Pseudostellaria, also known as baby ginseng, is a precious Chinese medicinal material, which is mostly planted in Fujian, Guizhou, Jiangsu, Anhui, Shandong,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 吴林坤陈军林文雄吴红淼林生
Owner FUJIAN AGRI & FORESTRY UNIV
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