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Application of lawn pennywort herb liver-healing tablets in preparation of drug for inhibiting cell proliferation of osteosarcoma cells HOS

A technology for osteosarcoma cells and cell proliferation, which is applied in the field of preparation of Tianhuxian Yugan Tablets, can solve the problems of large dosage for patients, affect clinical application, and many impurities, and achieve the effect of reducing dosage

Inactive Publication Date: 2016-08-03
JINAN XINSHIDAI MEDICINE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the application of Tian Huan Yu Gan Tablets in the preparation of drugs for inhibiting the proliferation of human osteosarcoma cells HOS cells, and there is no report on the use of supercritical extraction in the extraction and preparation of Tian Hu Xian Yu Gan Tablets, while traditional water decoction The cooking method is rough and outdated, and there are many impurities, which lead to excessive dosage for patients, which is inconvenient to take, and seriously affects the clinical application of this product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 699g of coriander, 1398g of apricot leaves, 699g of sorrel, and 209g of scorpion grass, and add them to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 900min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.30g.

[0025] After testing, the content of oleanolic acid in the finished product is 6.45mg / tablet.

Embodiment 2

[0027] Take 699g of coriander, 1398g of apricot leaves, 699g of sorrel, and 209g of scorpion grass, and add them to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 1000min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.30g.

[0028] After testing, the content of oleanolic acid in the finished product is 4.98mg / tablet.

Embodiment 3

[0030] Take 699g of coriander, 1398g of apricot leaves, 699g of sorrel, and 209g of scorpion grass, and add them to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 60°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 800min, and the supercritical extract is obtained. The supercritical extract is added to starch and 70% ethanol to make granules, dried, and pressed into tablets to make 500 tablets, each weighing 0.30g.

[0031] After testing, the content of oleanolic acid in the finished product is 3.22mg / tablet.

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PUM

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Abstract

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to application of lawn pennywort herb liver-healing tablets in preparation of a drug for inhibiting cell proliferation of osteosarcoma cells HOS and a preparation method of the lawn pennywort herb liver-healing tablets.The lawn pennywort herb liver-healing tablets are prepared from 699 g of lawn pennywort herb, 1,398 g of herba pimpinellae, 699 g of creeping woodsorrel herb and 209 g of brooklet anemone roots.The lawn pennywort herb liver-healing tablets are prepared by means of supercritical extraction, and the content of oleanolic acid is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of Tianhuxian Yugan Tablets in the preparation of medicines for inhibiting the proliferation of osteosarcoma cells and HOS cells and the preparation method of Tianhuxian Yugan Tablets. Background technique [0002] The standard number of Tianhuxian Yugan Tablets is WS-10213 (ZD-0213)-2002, which is recorded in the Hepatobiliary Subvolume of the National Compilation of Chinese Patent Medicine Standards. It is made from 699g of coriander, 1398g of apricot leaves, 699g of sorrel, and 209g of tiger palm as raw materials. It has the effects of clearing away heat and detoxifying, soothing the liver and promoting gallbladder. [0003] In the prior art, there is no report on the application of Tian Huan Yu Gan Tablets in the preparation of drugs for inhibiting the proliferation of human osteosarcoma cells HOS cells, and there is no report...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61K9/20A61P35/00
CPCA61K36/23A61K9/2059A61K36/185A61K36/71A61K2236/37A61K2236/51A61K2300/00
Inventor 不公告发明人
Owner JINAN XINSHIDAI MEDICINE SCI & TECH
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