Preparation method and application of Huixinkang tablet in medicine for inhibiting cell proliferation of breast tumor cells C127
A technology of Huixinkang and Huixincao, which is applied in the direction of antineoplastic drugs, drug combinations, and medical preparations containing active ingredients, can solve the problems of large dosage for patients, rough process, and many impurities, so as to reduce dosage, There is no bitterness in the dosage and the effect of taking a small dosage
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Embodiment 1
[0031] Take 780g of Uncaria vine, 910g of Huixincao, 26g of Polygonum multiflorum, 260g of Astragalus, 26g of Radix Notoginseng, 650g of Hawthorn, 260g of Licorice. 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 200min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, pulverized, added 10L of 60% ethanol, and put into a microwave extraction device for microwave extraction, with an extraction power of 700W, for 2 extractions. Each time for 8 minutes, the extracts were combined, concentrated, added to a D101 macroporous adsorption resin column, eluted with 60% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain a micr...
Embodiment 2
[0034] Take 780g of Uncaria vine, 910g of Huixincao, 26g of Polygonum multiflorum, 260g of Astragalus, 26g of Radix Notoginseng, 650g of Hawthorn, 260g of Licorice. 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, pulverized, added with 10L of 60% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 600W, and the extraction is performed twice. Combine extracts for 5 minutes each time, concentrate, add to D101 macroporous adsorption resin column, elute with 60% ethanol, collect 5 times column volume eluate, recover ethanol under reduced pressure, concentrate and dry to obtain microwave ext...
Embodiment 3
[0037] Take 780g of Uncaria vine, 910g of Huixincao, 26g of Polygonum multiflorum, 260g of Astragalus, 26g of Radix Notoginseng, 650g of Hawthorn, 260g of Licorice. 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 6%, the extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 220min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, crushed, added 10L of 60% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 800W, and the extraction is performed twice. For 10 minutes each time, combine the extracts, concentrate, add to the D101 macroporous adsorption resin column, elute with 60% ethanol, collect 5 times the column volume eluate, recover ethanol under reduced pressure, concentrate and dry to obtain the mic...
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