Preparation method of Qingmei cold tablet and application of Qingmei cold tablet in drugs for inhibiting anus sarcoma cell S-180 from cell proliferation
A green plum cold tablet and extraction technology, which is applied in the direction of antineoplastic drugs, drug combinations, and medical preparations containing active ingredients, etc., can solve the problems of large dosage for patients, rough process, and many impurities, and achieve small dosage and high dosage. No bitterness, effect of reducing dosage
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Embodiment 1
[0030] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 200min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, crushed, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 700W, and the extraction is performed twice. For 8 minutes each time, combine the extracts, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under reduce...
Embodiment 2
[0033] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent, the extraction pressure is 30MPa, the temperature is 50°C, and CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, crushed, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 600W, and the extraction is performed twice. Combine the extracts for 5 minutes each time, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under reduced pressure...
Embodiment 3
[0036] Take 200g of Artemisia annua, 267g of Five Fingers Mandarin, 134g of fenugreek, 200g of dog liver dish, 134g of licorice, 134g of Centella asiatica, 267g of Gangmei, and add Artemisia annua and lavender to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 6%, the extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 220min, and the supercritical extract is obtained for use; the rest of the traditional Chinese medicine is taken, pulverized, added 5L of 50% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 800W, and the extraction is performed twice. For 10 minutes each time, combine the extracts, concentrate, add to the D101 macroporous adsorption resin column, elute with 50% ethanol, collect 5 times the column volume eluate, recover ethanol under re...
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