The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method and an application of Danshilitong tablets, wherein the Danshilitong tablets are prepared by adopting 225 g of dichondra repens forst, 225 g of artemisiacapillaris thunb, 90 g of curcuma aromatica salisb, 150 g of tangerine peel, 45 g of scutellaria baicalensis georgi, 35 g of boswellia carteri, 80 g of mirabilite, 35 g of alunite, 45 g of rheum, 45 g of common burreed tuber, 45 g of cape jasmine, 25 g of licorice root, and 35 g of myrrh as bulk drugs. According to the prevent invention, ultra-fine crushing, supercritical fluid extraction, microwave extraction and macroporous resin absorption are performed to prepare the Danshilitong tablets, such that the hesperidin content is substantially increased. The present invention further provides the application of the Danshilitong tablets in preparation of mouse lymphoid neoplasm cell P388D1 proliferation inhibition drugs.