Application of Tiezhou Qingzhuo tablets to preparation of medicine for inhibiting cell proliferation of melanoma cell B16

A technology of iron broom clearing turbidity and cell proliferation, applied in the directions of drug combination, anti-tumor drugs, pharmaceutical formulations, etc., can solve the problems of large dosage of patients, affecting application, and many impurities, so as to achieve no bitterness in dosage, reduce dosage, easy to accept effect

Inactive Publication Date: 2014-04-02
徐桂娟
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the application of Tiezhuo Qingzhuo Pills in the preparation of drugs for inhibiting the proliferation of melanoma cell B16 cells, and there is no report on the use of supercritical and microwave technology in the extraction and preparation of Tiezhuo Qingzhuo Pills. However, the methods of direct crushing, percolation, extraction of volatile oil, and decoction in water are rough and outdated, and there are many impurities, which lead to excessive dosage for patients and are inconvenient to take, which seriously affects the clinical application of this product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 417g of pig scorpion, 417g of iron broom, 333g of fennel, 417g of Houttuynia cordata, 417g of dandelion, 250g of black ant, 417g of foreknowledge, 250g of plantain seed, 250g of poria cocos, 250g of yam, 167g of puzzle, 250g of dodder seed, and shayuanzi 250g, 333g of golden cherry root, 125g of polygala, 50g of licorice, add iron broom, black ant, houttuynia cordata, puzzle to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer to the total extraction solvent is 5%, the extraction pressure is 25MPa, the temperature is 40°C, and the CO 2 The flow rate is 2ml / g crude drug·min, the extraction time is 350min, and the supercritical extract is obtained for later use; the rest of the traditional Chinese medicine is taken, pulverized into a coarse powder, added with 15L of 30% ethanol, and put into a microwave extraction device for microwave extraction with an extraction power of 700W. 2 times, 20 minutes each t...

Embodiment 2

[0027] Take 417g of pig scorpion, 417g of iron broom, 333g of fennel, 417g of Houttuynia cordata, 417g of dandelion, 250g of black ant, 417g of foreknowledge, 250g of plantain seed, 250g of poria cocos, 250g of yam, 167g of puzzle, 250g of dodder seed, and shayuanzi 250g, 333g of golden cherry root, 125g of polygala, 50g of licorice, add iron broom, black ant, houttuynia cordata, puzzle to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1ml / g crude drug·min, the extraction time is 370min, and the supercritical extract is obtained for later use; the rest of the traditional Chinese medicine is taken, pulverized into a coarse powder, added with 15L of 60% ethanol, and put into a microwave extraction device for microwave extraction, with an extraction power of 600W. 2 times, 15 minutes each time, c...

Embodiment 3

[0030] Take 417g of pig scorpion, 417g of iron broom, 333g of fennel, 417g of Houttuynia cordata, 417g of dandelion, 250g of black ant, 417g of foreknowledge, 250g of plantain seed, 250g of poria cocos, 250g of yam, 167g of puzzle, 250g of dodder seed, and shayuanzi 250g, 333g of golden cherry root, 125g of polygala, 50g of licorice, add iron broom, black ant, houttuynia cordata, puzzle to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3ml / g crude drug·min, the extraction time is 3300min, and the supercritical extract is obtained for later use; the rest of the traditional Chinese medicine is taken, pulverized into coarse powder, added with 15L of 60% ethanol, and put into a microwave extraction device for microwave extraction. The extraction power is 800W. 2 times, 25 minutes each time, c...

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Abstract

The invention belongs to the technical field of traditional Chinese medicines and in particular relates to application of Tiezhou Qingzhuo tablets to preparation of a medicine for inhibiting cell proliferation of melanoma cell B16 and a preparation method of the Tiezhou Qingzhuo tablets. According to the application of the Tiezhou Qingzhuo tablets to preparation of the medicine for inhibiting cell proliferation of melanoma cell B16, the Tiezhou Qingzhuo tablets are prepared from the following raw material medicines: 417 g of cleavers, 417 g of Lespedeza cuneata, 333 g of rhizoma dioscoreae hypoglaucae, 417 g of Houttuynia cordata, 417 g of dandelion, 250 g of black ant, 417 g of Akebia Fruit, 250 g of semen plantaginis, 250 g of Tuckahoe, 250 g of Chinese yam, 167 g of Alpinia oxyphylla, 250 g of semen cuscutae, 250 g of flatstem milkvetch seed, 333 g of cherokee rose root, 125 g of polygala tenuifolia and 50 g of liquorice, and are prepared by supercritical extraction and microwave extraction, so that the diosgenin content is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of Tiezhuo Qingzhuo Tablet in the preparation of medicines for inhibiting the proliferation of melanoma cell B16 cells and the preparation method of Tiejiao Qingzhuo Tablet. Background technique [0002] The standard number of Tiexuan Qingzhuo Pills is WS-10190 (ZD-0190) 2002, which is recorded in the National Compilation of Chinese Patent Medicine Standards, Internal Medicine, Surgery and Gynecology. 417g of pig scorpion, 417g of iron broom, 333g of fennel, 417g of Houttuynia cordata, 417g of dandelion, 250g of black ant, 417g of foreknowledge, 250g of plantain seed, 250g of poria cocos, 250g of yam, 167g of puzzle, 250g of dodder seed, and sand garden seed 250g, 333g of golden cherry root, 125g of polygala, and 50g of licorice are made as raw materials, which have the effects of clearing away heat and detoxification, promoting d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9062A61K9/20A61P35/00A61K35/64
Inventor 孙亚平
Owner 徐桂娟
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