Standard gene for identifying pterocarpus santalinus molecules and molecular identification method
A lobular rosewood and standard gene technology, applied in the field of molecular biology, can solve the problems of sample damage, unguaranteed identification accuracy, complicated operation process, etc.
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[0012] In the experiment, 5 samples of Pterocarpus lobosa from different sources were used to extract the sample DNA with the DNA extraction kit from TIANGENE Company. Forward primer used: TTAGCCTTTGTTTGGCAAG, reverse primer: AGAGTTTGAGAGTAAGCAT, PCR amplification conditions were pre-denaturation at 94°C for 4 min, followed by denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 1.5 min. A total of 35 cycles were performed. Finally, extend at 72°C for 10 min. The obtained products were sequenced and compared, and the homology between the samples was maintained at more than 99%, and the obtained sequences were compared with other species online, and the homology was below 52%.
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