Standard gene for identifying pterocarpus santalinus molecules and molecular identification method
A lobular rosewood and standard gene technology, applied in the field of molecular biology, can solve the problems of sample damage, unguaranteed identification accuracy, complicated operation process, etc.
Inactive Publication Date: 2016-08-10
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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Problems solved by technology
Nowadays, microscopic anatomy and fluorescence observation methods are mostly used to identify Pterocarpus lobularis, the operation process is complicated, and
Method used
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[0012] In the experiment, 5 samples of Pterocarpus lobosa from different sources were used to extract the sample DNA with the DNA extraction kit from TIANGENE Company. Forward primer used: TTAGCCTTTGTTTGGCAAG, reverse primer: AGAGTTTGAGAGTAAGCAT, PCR amplification conditions were pre-denaturation at 94°C for 4 min, followed by denaturation at 94°C for 45 s, annealing at 50°C for 45 s, and extension at 72°C for 1.5 min. A total of 35 cycles were performed. Finally, extend at 72°C for 10 min. The obtained products were sequenced and compared, and the homology between the samples was maintained at more than 99%, and the obtained sequences were compared with other species online, and the homology was below 52%.
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Pterocarpus santalinus is also known as a red sandalwood and is an apapilionaceae pterocarpus plant. Because that material quality is good, the distribution area is small, a material forming cycle is long, and the market prices are soaring, pterocarpus santalinus counterfeits spread unchecked under the huge benefit drive, and how to accurately identify the pterocarpus santalinus becomes the biggest problem in the pterocarpus santalinus trading market. The invention provides a standard gene for identifying pterocarpus santalinus molecules and a molecular identification method. The method is applied to pterocarpus santalinus identification and has the advantages of being quick and accurate, bringing small sample damages and the like.
Description
technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a standard gene for plant molecular identification and a molecular identification method. Background technique [0002] Small-leaved red sandalwood (Pterocarpus santalinus), also known as sandalwood red sandalwood, is a plant of the genus Pterocarpus in the family Papilionaceae. Small-leaf red sandalwood is solid and thick, with fine wood, high density, small brown eyes, excellent stability, beautiful texture, good toughness, and resistance to carving. It is produced in narrow areas in and around southern India. Small-leaf red sandalwood is deep purple in color. It has always been a special wood for royal and noble furniture. It is expensive and ranks first among all woods. It is called "the king's wood". The furniture used by the French Royal Louis XVIII in the 18th century was also made of high-quality small-leaf red sandalwood. Among the mahogany species identifie...
Claims
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Login to view more IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/166
Inventor 王兵益杨时宇廖礼彬李体初
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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