This invention provides a
tissue culture medium for *
Pandanus tectorius* leaves and a method for rapid propagation through
tissue culture. Using young lateral buds of *
Pandanus tectorius* leaves as explants is preferable due to its low
contamination rate, rapid
initiation of proliferation, and superior surface sterilization method, resulting in low
contamination and
browning. Adding 0-0.1 mg TDZ and 1-3 mg 6-BA to MS medium for *
Pandanus tectorius* leaf
bud induction culture rapidly initiates proliferation. Subculture with NAA and 6-BA in MS medium effectively improves the
proliferation rate, producing robust and rapidly growing seedlings that effectively balance
callus amplification and
bud propagation. Adding NAA to 1 / 2 MS medium for *Pandanus tectorius* leaf rooting culture results in high rooting efficiency and good
root growth and development. This allows for rapid and large-scale propagation of *Pandanus tectorius* leaf
tissue culture seedlings, enabling large-scale production, promoting the vigorous development of *Pandanus tectorius* leaf cultivation, and stimulating the development of related industries such as pharmaceuticals, health foods, and ornamental flowers based on *Pandanus tectorius* leaf raw materials.