Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of preparation method of water-soluble nanoparticles
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A nanoparticle and polymer technology, applied in the field of biopharmaceuticals and nanometers, can solve the problems of water-soluble phase difference, low bioavailability, and high drug loading, and achieve low toxicity, good biocompatibility, and strong targeting effects
Inactive Publication Date: 2019-03-26
BEIJING FORESTRY UNIVERSITY
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] The purpose of the present invention is to address the deficiencies in the prior art and provide a new method for preparing water-soluble nanoparticles. PET-UA can self-assemble into nanoparticles in an aqueous medium to solve the problem of UA's water-soluble phase difference and low bioavailability. The problem is that the self-assembly process encapsulates DHA or HCPT at the same time to form PET-UA(DHA)·NPs and PET-UA(HCPT)·NPs, which increases the drug loading capacity of the system, and the encapsulated drugs have a wide choice, a large number of hydrophobic Drugs are self-assembled and packaged at the same time. The particle size of the nanoparticles is not affected much before and after the packaged drugs. Pectin is used as the drug carrier, which has good targeting and high drug loading, and avoids incomplete release of drugs and Toxic and side effects of sudden release on patients
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0022] Embodiment 1, a kind of preparation method of novel water-soluble nanoparticles:
[0023] (1) Acetylation of PET
[0024] Take 1g of PET (the degree of esterification is 50%~80%), slowly add 25ml of glacial acetic acid, 15ml of acetic anhydride and 0.6ml of sulfuric acid, react in ice bath for a period of time, then raise the temperature to 50°C, stir until the solution is clear, and the mixture is rotary evaporated , washed with ethanol until neutral, freeze-dried to obtain acetylated pectin powder A.
[0025] (2) Synthesis of PET-UA
[0026] Take 0.1g of PET in (1), slowly add it into a 25ml round bottom flask containing 4ml of pyridine, and sonicate for 10min, stir and dissolve for 20min, then add 0.1g of activator 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide hydrochloride (EDC), 0.02g catalyst 4-dimethylaminopyridine (DMAP), stirred for 30min, added 0.1g of UA, and reacted in water bath for 48h to obtain a PET-DHA polymer mixture.
[0027] (3) Purification of P...
Embodiment 2
[0031] Embodiment 2, a kind of preparation method of novel water-soluble nanoparticles:
[0032] In this example, through the same synthesis method and steps as in Example 1, PET 0.1g, UA 0.5g, the measured particle size is 20-80nm.
Embodiment 3
[0033] Embodiment 3, a kind of preparation method of novel water-soluble nanoparticles:
[0034] In this example, through the same synthesis method and steps as in Example 1, PET 0.1g, UA 0.05g, the measured particle size is 30-130nm.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
radius
aaaaa
aaaaa
particle diameter
aaaaa
aaaaa
particle diameter
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a preparation method of novel water-soluble nanoparticles. The method comprises the following steps: pectin (PET) is used as a carrier; esterification is carried out between carboxyl of pectin and hydroxy of ursolic acid (UA) in order to form macromolecule micelle; self assembly is carried out in order to form nanoparticles PET-UA.NPs, a dihydroartemisinin hydrophobic medicament or 10-hydroxycamptothecin is enveloped during the process of self assembly, in order to form PET-UA(DHA).NPs or PET-UA(HCPT).NPs, and the nanoparticles with core-shell structures are formed. The water-soluble nanoparticles are used as a nanometer medicament with a slow release function, and the nanoparticles have the advantages of adjustable drug loading, good targeting ability, good stability, good biocompatibility, and low toxicity. The nanoparticles belong to the fields of biological pharmacy and nanometer technology, and the method has the advantages of simple preparation technology, operation convenience, and short period.
Description
technical field [0001] The invention belongs to the fields of biopharmaceuticals and nanotechnology, relates to a drug delivery system, and in particular to a preparation method of novel water-soluble nanoparticles. Background technique [0002] Ursolic acid (UA for short) and dihydroartemisinin (DHA for short) are insoluble in water, and direct oral administration has a certain degree of toxic and side effects on the body. They cannot treat a single cancer and have no effect on cancer cells. Recognition ability, killing cancer cells while also killing normal cells, and poor bioavailability, these limit the wide application of UA and DHA in the pharmaceutical field, therefore, to solve the problem of its water solubility, increase drug stability, Improving the slow-release killing ability of a single cancer cell is the key to the clinical application of DHA. [0003] Pectin is a polysaccharide macromolecular substance present in plant cell walls. Widely present in apples, ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
