Special-effect stem-cell in-vitro induction technology

A stem cell and stem cell factor technology, applied in the field of bioengineering, can solve problems such as the unsatisfactory target homing rate of stem cells and the low utilization rate of stem cells.

Inactive Publication Date: 2016-12-21
SHANGHAI JIAOJIAREN MEDICAL COSMETOLOGY OUT PATIENT DEPT CO LTD
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  • Application Information

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Problems solved by technology

At present, in the current in vitro induction technology of stem cells in China, the target homing rate of stem cells in vivo is not very ideal, so the utilization rate of stem cells is not very high. If a new type of special effe

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Embodiment Construction

[0010] The present invention will be described in detail below in conjunction with specific embodiments.

[0011] A technique for in vitro induction of special-effect stem cells, which specifically includes the following steps: 1) Isolation and cultivation of human umbilical cord stem cells: In this experiment, human umbilical cord stem cells were used, human umbilical cord Wharton's jelly was taken aseptically, and DMEM culture medium containing 12% FBS was used repeatedly Rinse Wharton's jelly, collect the cell suspension, and change the medium once every 24 hours and 48 hours of the primary culture; and then primary culture for about 7 days, if the cells are observed to form sheets, digest with 0.3% trypsin and passage to a new bottle , after standing in the incubator for 40 minutes, turn the bottom of the bottle up, blow gently, and discard the suspended cells (mainly epithelial cells), while most of the human umbilical cord stem cells firmly adhere to the wall; add the who...

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Abstract

The invention discloses a special-effect stem-cell in-vitro induction technology. The special-effect stem-cell in-vitro induction technology includes: separating and culturing human umbilical cord stem cells, to be more specific, using the human umbilical cord stem cells, taking human umbilical cord Wharton's jelly in a sterile manner, collecting cell suspension, changing the suspension when primary culture is performed for 24 hours, and changing the suspension when the primary culture is performed for 48 hours; performing primary culture for 7 days, digesting with trypsin if cell flocking is observed and then performing passage into a new bottle, and discarding suspended cells, wherein most of the human umbilical cord stem cells firmly attach to the bottle wall; adding a whole culture medium to start passage culture, changing the culture liquid every 3 days, and performing continuous passage for 3 generations; performing in-vitro human umbilical cord stem cell induction, to be more specific, selecting the stem cells with appropriate passage time and within 3 generations, changing the liquid, adding an induction differentiation agent 5-azacytidine, adding a liver cell growth factor HGF, basic growth factors EGF+bFGF and a stem cell factor SGF, and performing in-vitro induction for 24 hours. By the latest-generation in-vitro induction technology, the target homing rate of the in-vivo stem cells is 100%.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a special-effect stem cell induction technology in vitro. Background technique [0002] Stem cells are a kind of pluripotent cells with self-replication ability, which can differentiate into various functional cells under certain conditions. According to the developmental stage of stem cells, they are divided into embryonic stem cells and adult stem cells; according to their developmental potential, stem cells are divided into three categories: totipotent stem cells, pluripotent stem cells and multipotent stem cells. Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. [0003] Human umbilical cord stem cells come from newborns. They are convenient to obtain, easy to expand in vitro, safe to use, free from virus in...

Claims

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Application Information

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IPC IPC(8): C12N5/074
CPCC12N5/0662C12N2501/06C12N2501/115C12N2501/12C12N2501/125C12N2509/00
Inventor 王浩
Owner SHANGHAI JIAOJIAREN MEDICAL COSMETOLOGY OUT PATIENT DEPT CO LTD
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