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A rapid detection method for highly poisonous mushrooms

A detection method and mushroom technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of cumbersome detection process, poor usability, dependence, etc.

Active Publication Date: 2019-02-15
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thin-layer chromatography detection is difficult and time-consuming, requires standard products and has high requirements for operators
The detection process of high-performance liquid chromatography is cumbersome, time-consuming and dependent on expensive equipment, and the ease of use is the worst
McFarland's color reaction is easy to operate, but it needs to use 36-38% fuming concentrated hydrochloric acid, which is dangerous to operate and cannot be used in the field and in a non-ventilated environment
[0004] At present, there is no relevant report on the detection of amanitin with low concentration hydrochloric acid in the prior art, and there is no report on the detection method of amanitin at a safe concentration of hydrochloric acid

Method used

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  • A rapid detection method for highly poisonous mushrooms
  • A rapid detection method for highly poisonous mushrooms
  • A rapid detection method for highly poisonous mushrooms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The present invention describes a color reaction-based rapid detection method for highly toxic mushrooms, aiming at the main deadly mushroom toxin - amanitin. The method of the invention is simple and quick to operate, and uses wood (birch, pine, etc.) or newsprint to identify the highly poisonous mushrooms containing amanitin through color reaction under the action of medium and low concentration hydrochloric acid. The following examples use birch wood and low-concentration hydrochloric acid (5% and 10%) as materials to describe this identification process in detail, and describe each step completely and accurately. The whole experimental process is roughly divided into two steps of material preparation and color development, the first step takes 5-8 minutes, the second step (color development) takes 2-5 minutes when heated, and 15-25 minutes at room temperature . The specific experimental conditions used in this example are heating at 55° C. for 5 minutes, material p...

Embodiment 2

[0053] 1. Materials and methods

[0054] This embodiment is completely consistent with embodiment 1 in materials and methods, and the only difference is that the selected highly poisonous mushroom objects are different, as follows:

[0055] Select fresh Amanita, Galerina, Lepiota or other mushrooms containing Amanitin that are stored at -80°C. In this example, Amanita is specifically used. A representative highly poisonous species in Amanita, deadly Amanita (A.exitialis), has a large number of poisoning death cases reported in southern China, especially in Guangdong.

[0056] 2. Results and analysis

[0057] 2.1 Color reaction between highly poisonous amanita and birch powder

[0058] experiment result shows( figure 2 ), Amanita (Amanita) or genus Galerina (Galerina) or Lepiota (Lepiota) or other mushrooms containing Amanitin (Amanitin), specifically to the present embodiment is deadly Amanita (A.exitialis ), under the joint action of low-concentration hydrochloric acid a...

Embodiment 3

[0060] 1. Materials and methods

[0061] This embodiment is completely consistent with embodiment 1 in materials and methods, and the only difference is that the selected highly poisonous mushroom objects are different, as follows:

[0062] Select fresh Amanita (Amanita) or Galerina (Galerina) or Lepiota (Lepiota) or other mushrooms containing Amanitin (Amanitin) preserved at -80 ℃. A representative highly poisonous species, G. marginata, has been reported to be poisoned in the world and in China, but due to its small size and low toxin content, there are very few fatal cases. The highly poisonous mushroom selected in this example is based on the following considerations: the toxin content of the mushroom sample collected by the research group is low, about 1 / 5 of that of Amanita amanita, and the toxin content is the lowest among the mushrooms collected by the research group. This mushroom was used to test the sensitivity of the method of the present invention. If the detect...

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Abstract

The invention provides a simple and rapid method for identifying extremely toxic mushrooms such as Amanita, Galerina, Lepiota, other mushrooms containing Amanitin, and the like. The method can also be used to detect pure Amanitin. Amanitin and lignin form a blue-green substance with a prominent characteristic in the presence of hydrochloric acid, and through the color development reaction, extremely toxic mushrooms can be rapidly identified within 10 to 30 minutes. The experiment results show that when the hydrochloric acid concentration is 5 to 15%, wood (birch, pine, etc.) can be used to effectively detect Amanitin, MgCl2 can improve the color developing effect; when the hydrochloric acid concentration is 15 to 25%, newspaper can effectively detect Amanitin, and the effect is better, when MgCl2 exists in the solution. The provided method can rapidly and accurately detect Amanita, Galerina, Lepiota, and other mushrooms containing Amanitin, and has an important meaning for mushroom poisoning prevention, understanding of mushroom poisoning reason, and treatment design.

Description

[0001] Field: [0002] The invention belongs to the field of biotechnology, and in particular relates to a rapid detection method for highly poisonous mushrooms. The method uses low-concentration hydrochloric acid to react with wood (birch, pine, etc.) or newsprint to generate a blue-green product with obvious characteristics. Rapid identification of highly poisonous mushrooms containing amanitin in minutes. Background technique: [0003] Among the macrofungi, the mushrooms of the Amanitaceae family (Amanitaceae) and especially the genus Amanita (Amanita) are the most conspicuous. Most of the mushrooms of the genus Amanita are obligate ectomycorrhizal fungi, which have a promising application prospect in barren hill afforestation and vegetation restoration; some Amanita mushrooms are well-known edible fungi with important economic value (Yang Zhuliang 2005). What must be paid attention to is that in the genus Amanita group (A.sect.Phalloideae), all species contain highly toxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 罗宏李萱乔治文杨祝良
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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