A Method for Quickly Observing Arabidopsis Ovule Morphology and Counting the Number of Outer Integument Cells

Abstract

The invention provides a method of quickly observing ovule morphology and counting number of outer integument cells of arabidopsis thaliana. In the method, ovules of the arabidopsis thaliana are treated with a fluorescent bleaching agent, so that the number of the integument cells of ovules of the arabidopsis thaliana can be counted quickly, rapidly and timely under a confocal microscopy and an ordinary optical microscope. Compared with a common section and interference contrast-transparence method, the method is simple and has better effect.

Description

technical field

[0001] The invention belongs to the field of biology and relates to a method for quickly observing the morphology of Arabidopsis ovules and counting the number of outer integument cells. Background technique

[0002] The ovule is the female reproductive organ of a plant, and it is the structure that develops into a seed after fertilization. Therefore, whether the ovule develops normally directly determines whether the plant can form seeds and reproduce offspring. The mature ovule is composed of several specialized parts, including the sporophytic tissue (nucellus, integument, nucellus) and the female gametophytic tissue (embryo sac). Gymnosperms generally have only one integument structure covering the nucellus to form a closed structure, while the ovules of angiosperms usually include two integument structures, that is, the nucellus and the embryo sac are surrounded by the outer integument and the inner integument (Kelley and Gasser , 2009). After the ovul...

Examples

Embodiment 1

[0019] A method for quickly observing Arabidopsis ovule morphology and counting the number of outer integument cells, the steps are as follows:

[0020] (1) Solution preparation: prepare high-pressure sterilized double-distilled water, and then use high-pressure sterile water to prepare 1‰w / v CALCOFLOUR WHITE fluorescent whitening agent solution (Cat.No. C-CLFR10) and A solution of potassium hydroxide with a concentration of 2M;

[0021] (2) Obtaining materials: Take the buds of Arabidopsis thaliana that are just about to turn white and put them on a glass slide with double-sided adhesive tape. The carpel of the mustard pistil is cut from the middle, and finally the ovules on the placenta are removed with a needle;

[0022] (3) Glass slide preparation: use a 10µL pipette gun to draw 10µL of 1‰w / v fluorescent whitening agent and place it on a glass slide, then place the ovules and placenta obtained above on the fluorescent whitening agent, and then Add 10 µL of 2M potassium h...

technical field

[0001] The invention belongs to the field of biology and relates to a method for quickly observing the morphology of Arabidopsis ovules and counting the number of outer integument cells. Background technique

[0002] The ovule is the female reproductive organ of a plant, and it is the structure that develops into a seed after fertilization. Therefore, whether the ovule develops normally directly determines whether the plant can form seeds and reproduce offspring. The mature ovule is composed of several specialized parts, including the sporophytic tissue (nucellus, integument, nucellus) and the female gametophytic tissue (embryo sac). Gymnosperms generally have only one integument structure covering the nucellus to form a closed structure, while the ovules of angiosperms usually include two integument structures, that is, the nucellus and the embryo sac are surrounded by the outer integument and the inner integument (Kelley and Gasser , 2009). After the ovul...

Examples

Embodiment 1

[0019] A method for quickly observing Arabidopsis ovule morphology and counting the number of outer integument cells, the steps are as follows:

[0020] (1) Solution preparation: prepare high-pressure sterilized double-distilled water, and then use high-pressure sterile water to prepare 1‰w / v CALCOFLOUR WHITE fluorescent whitening agent solution (Cat.No. C-CLFR10) and A solution of potassium hydroxide with a concentration of 2M;

[0021] (2) Obtaining materials: Take the buds of Arabidopsis thaliana that are just about to turn white and put them on a glass slide with double-sided adhesive tape. The carpel of the mustard pistil is cut from the middle, and finally the ovules on the placenta are removed with a needle;

[0022] (3) Glass slide preparation: use a 10µL pipette gun to draw 10µL of 1‰w / v fluorescent whitening agent and place it on a glass slide, then place the ovules and placenta obtained above on the fluorescent whitening agent, and then Add 10 µL of 2M potassium h...

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