Glaucoma diagnosis molecular marker lncRNAs NR_026887, kit and application

A molecular marker, glaucoma technology, applied in the field of long-chain non-coding RNA markers, which can solve problems such as visual impairment

Active Publication Date: 2017-07-28
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these tests can diagnose glaucoma, studies have shown that more than 50% of pati...

Method used

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  • Glaucoma diagnosis molecular marker lncRNAs NR_026887, kit and application
  • Glaucoma diagnosis molecular marker lncRNAs NR_026887, kit and application
  • Glaucoma diagnosis molecular marker lncRNAs NR_026887, kit and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Expression profiles of lncRNAs and mRNAs in aqueous humor of glaucoma patients:

[0035] 1.1 Materials and reagents

[0036] 1.1.1 Main Instruments

[0037] Commonly used centrifuges and cryogenic centrifuges were purchased from Eppendorf; high-speed centrifuges were purchased from Beckman; Real-Time PCR instruments were purchased from ABI; pipettes and electric pipettes were purchased from Eppendorf; Q5000 was purchased from Quawell Technology; The ice machine was purchased from Sanyan Company.

[0038] 1.1.2 Materials and reagents

[0039] Different types of centrifuge tubes and PCR tubes were purchased from Axygen; DEPC water was purchased from Dingguochangsheng; Trizol was purchased from Invitrogen; TargetAmp TMThe 1-Round aRNA Amplification Kit was purchased from Epicentre; the Transcriptor First Strand cDNA Synthesis Kit was purchased from Roche; the Quick AmpLabeling Kit, One-Color Kit was purchased from Agilent Technologies; the human lncRNAs micro...

Embodiment 2

[0063] Example 2: The correlation between specific lncRNAs and mRNAs in aqueous humor is clarified by CNC analysis

[0064] In order to further explore the possible functions of lncRNAs expressed in the aqueous humor of glaucoma patients, we used CNC (coding-noncoding gene co-expression) to analyze lncRNAs that were significantly correlated with the expression of mRNAs of glaucoma-related genes. CNS analysis is an analysis method that links lncRNAs to mRNAs through lncRNAs and mRNA co-expression data. Through CNC analysis, mRNAs with the same expression pattern as a certain lncRNA can be found. Through the functions of these mRNAs, lncRNAs can be associated with specific signaling pathways or disease conditions, so as to facilitate the prediction of the function of lncRNAs and reveal their mechanism of action.

[0065] 2.1 Method

[0066] Select the mRNAs that are differentially expressed in the aqueous humor and correlated with the occurrence and development of glaucoma, and...

Embodiment 3

[0070] We used qRT-PCR in the aqueous humor of patients with glaucoma and age-related cataract to verify that among the 10 lncRNAs in Example 2, the differential expression levels of 7 lncRNAs in the two types of aqueous humor were statistically different, and the differential expression of 3 lncRNAs in the two types of aqueous humor There was no statistical difference in differential expression. Then select lncRNAs with statistical difference: ENST00000564363, NR_026887, TCONS_00025577 and ENST00000508241 for further verification.

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Abstract

The invention discloses a molecular marker lncRNAs NR_026887 for glaucoma diagnosis, a kit and application. The inventor finds that relative to the control group, expression of lncRNAs:NR_026887 in aqueous humour of glaucoma patients is up-regulated., thus hinting that NR_026887 is high expression lncRNAs (long noncoding RNA) of glaucoma and is a biomarker conducive to glaucoma diagnosis. The molecular marker provided by the invention provides a powerful molecular biological basis for diagnosis of glaucoma, and has far-reaching clinical significance and generalization performance.

Description

[0001] Technical field: [0002] The invention relates to the technical field of molecular diagnosis, in particular to a long-chain non-coding RNA marker, a kit and an application that are helpful for the diagnosis of glaucoma. [0003] Background technique: [0004] The eye is a very important sensory organ of the human body, which can receive external light stimulation and transmit the light impulse to the brain center to cause vision. Da Vinci once said: "The eyes are the windows of the soul. Through the eyes, people can embrace and appreciate the infinite beauty of the world, and the soul can live in the body." In the information age, about 90% of the information that people get from the outside world through sensory organs is completed by eyes. According to the data of the World Health Organization, ophthalmic diseases have become the third most harmful and affecting people's quality of life after tumors and cardiovascular diseases. Among all ophthalmic diseases, glaucom...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 江冰谢犁犁张璐丝段宣初石晶明刘可陈慧慧
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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