39 results about "Aqueous humour" patented technology

A device is disclosed for selectively improving and/or permanently ensuring the permeability for ocular aqueous humour through the trabecular formations into Schlemm's canal. A small tubular element is provided, whose wall material encloses a hollow duct that presents an open configuration on both ends along the longitudinal extension of the hollow duct, so that the size and the shape of the small tubular element correspond approximately to the internal contour of Schlemm's canal, and the wall material as well as the wall thickness are so selected that upon introduction into Schlemm's canal the small tubular element keeps the canal open and dilates adjacent trabecular formations.

Described herein is a device for glaucoma treatment and monitoring that comprises a combination of an intraocular pressure (IOP) sensor and a glaucoma drainage device. The device comprises an IOP sensor and an inductive antenna mounted within a porous biocompatible material that forms the drainage path. The IOP sensor is mounted in a footplate portion of the device and is mountable in the anterior chamber of an eye. The footplate portion is connected to a body portion that houses the spiral antenna by a neck portion which retains the footplate portion in a suitable position within the anterior chamber. Due to its size, the footplate portion housing the IOP sensor can readily be inserted into the anterior chamber with the body portion housing the spiral antenna located outside of the anterior chamber in a sub-scleral space to disperse the aqueous humour.

A drain valve (10) implantable surgically in the eye (O) of a patient for the treatment of glaucoma, comprising a main body (11), and a drainage tube (12), connected at one end with the main body (11) and provided to be implanted in the eye (O) so as to penetrate with its distal tip inside the anterior chamber (CA) of the eye globe (GO), so as to allow the drainage outwards of the aqueous humour (UA), wherein the valve (10) is characterised by a series of significant improvements, including: 1) an extractable configuration (12a, 12b) of the drainage tube (12), to allow the adaptation (f1) of the length of the drainage tube (12) during the surgical operation to implant the valve (10); 2) a flattened or flat shape in section (12′) of the drainage tube (12, 12a, 12b) suitable for reducing the dimensions in the radial direction (IR) of the drainage tube (12) with respect to the surface of the eye globe (GO); 3) a connection free from encumbrances between the tip portion (12f) of the drainage tube (12), provided to penetrate inside the eye globe (GO, CA), and the remaining part (12b′) of the drainage tube (12); 4) a series of retaining bags or compartments (11b), formed along the lower or soffit surface (S″) of the main body (11) in contact with the surface of the eye globe (GO), wherein these retaining bags (11b) are associated with respective through holes (11a) extended through the same main body (11) of the valve and are suitable for receiving and retaining the aqueous humour (UA), so as to improve lubrication of the eyeball in the zone of the valve, once implanted; 5) a divergent fan-shaped opening (11c) formed on the extrados (S′), not in contact with the eye globe, of the main body (11) of the valve, in order to convey the aqueous humour (UA) and improve the lubrication around the drain valve (10), once implanted; and 6. a modified configuration, with respect to conventional drain valves, such as to allow, during the surgical operation, an arrangement, closer to the iris, of the holes (11d) for the insertion of the yarn for fixing the valve to the surface of the eye globe. Thanks to these improvements the drain valve (10) ensures considerably improved and superior performances with respect to the currently known drain valves in use in the medical field for treating glaucoma.

An implantable device for mechanically stimulating the trabecular meshwork is disclosed. The device is implanted in the eye adjacent the trabecular meshwork. The device imparts mechanical stimulating in the form of vibrations or movement to the trabecular meshwork. The imparted mechanical stimulation causes the trabecular meshwork to move in a manner that produces a pumping action to remove aqueous from the anterior chamber of the eye.

Method for the microbiological determination of traces of antibiotics in low volume biological samples, which comprises determining the minimum inhibitory concentration (MIC) of an antibiotic selected from among minocycline, ciprofloxacin, kanamycin, tetracycline and oxacillin against Staphylococcus aureus. The bacteria are grown in Mueller-Hinton broth (MHB) overnight, said cultures subsequently being diluted to 0.5 McFarland standard (1.5×108 cells/mL). Said antibiotics are subsequently added to 96-well plates in a final volume of 200 μL and incubated at 37° C. for 18 hours. Absorbency is determined and the results are expressed as the percentage of inhibition relative to the control containing the biological sample, in turn selected from urine, blood, synovial fluid, aqueous humour of the eye or cerebro spinal fluid (CSF), and the bacterial culture. The curve representing the percentage of inhibition of bacterial growth versus concentration of antibiotic is obtained, and is adjusted by the “Four Parameter Logistic Curve (4PL)”, yielding the concentration values of the antibiotics in the biological sample. The method determines amounts of antibiotic in the biological sample even at ng/mL level.

Provided is an anterior segment aqueous fluid circulating flow simulation device. The organization of the simulation device comprises a cornea (1), a sclera (6), a crystalline lens (12), a vitreous chamber (14), an iris (11) and the like. A functional mechanism comprises a aqueous fluid circulating flow control system, a crystalline lens moving control system, a pupil retardation simulation system and an intraocular temperature adjusting system which work in a cooperative mode, so that normal and abnormal aqueous fluid circulating flow simulation is guaranteed. An auxiliary device comprises an aqueous fluid discharging groove (2), an aqueous fluid flow-in groove (5), a control panel (9), a crystalline lens inner cavity (13), pipelines (3, 4, 23 and 24), a water-leakage-preventing rubber mat (17), an external temperature control system and the like.

The invention discloses a molecular marker lncRNAs NR_026887 for glaucoma diagnosis, a kit and application. The inventor finds that relative to the control group, expression of lncRNAs:NR_026887 in aqueous humour of glaucoma patients is up-regulated., thus hinting that NR_026887 is high expression lncRNAs (long noncoding RNA) of glaucoma and is a biomarker conducive to glaucoma diagnosis. The molecular marker provided by the invention provides a powerful molecular biological basis for diagnosis of glaucoma, and has far-reaching clinical significance and generalization performance.

The invention relates to a stainless steel device for permanent insertion into the sclerocorneal limbus, with different drainage and anchorage systems, enabling the aqueous humour to discharge through one or more virtual spaces from the anterior chamber to an intrascleral space in cases of simple glaucoma or to the equatorial episclera in cases of complex glaucoma, such as neovascular glaucoma. The space is virtual, not real, as in the case of the tubular device. It enables the aqueous humour to discharge slowly and continuously, minimizing the possibility of the chamber being flat or of germs penetrating through the device. Furthermore, the anchorage system is less aggressive, thus reducing the possibility of bleeding.

The invention provides a micro-fluidic cell culture device for simulating filter bubbles and application of the micro-fluidic cell culture device. The micro-fluidic cell culture device for simulating the filter bubbles comprises a cover plate, a cell culture main body and a notch groove bottom plate containing a micro-channel from top to bottom, the cell culture main body contains a semi-arc dome-shaped raised cell culture cavity with a flat bottom; and an inflow channel, an outflow channel and a gathering outflow channel are engraved on the grooving bottom plate. The invention also provides a use method of the micro-fluidic cell culture device for simulating the filtering bubble. The micro-fluidic cell culture device for simulating the filtering bubble simulates the biological form of the filtering bubble and the one-way and dynamic flowing process of aqueous humor in eyes, can be used for culturing cells in a state close to a natural physiological state and researching the functions of the cells, and has important significance on research of related diseases.

The invention discloses a multipurpose anterior chamber puncture device with a pressure monitoring function. The multipurpose anterior chamber puncture device comprises an aqueous humor collecting assembly, a pressure measuring and displaying module and a pressure/flow stepless inching adjusting module which are sequentially connected from front to back. Wherein the aqueous humor collecting assembly is used for puncturing the cornea of an eyeball and collecting aqueous humor; the aqueous humor collecting assembly is provided with scales for reading the release amount of the aqueous humor; the pressure measurement and display module is used for measuring and displaying anterior chamber intraocular pressure in real time when the aqueous humor collection assembly collects aqueous humor and guiding the pressure relief control process; the pressure/flow stepless inching adjusting module is used for adjusting intraocular pressure and the flow velocity of the aqueous humor when the aqueous humor collecting assembly collects the aqueous humor. According to the multi-purpose anterior chamber puncture device with the pressure monitoring function, quantitative collection of aqueous humor can be achieved, intraocular pressure can be monitored in real time, and the intraocular pressure release speed and the aqueous humor outflow speed can be controlled.

The invention belongs to the technical field of medical instruments and relates to a split type aqueous humor drainage device. The split type aqueous humor drainage device comprises a puncture needle, a drainage tube and a throttling structure; a needle head of the puncture needle penetrates through a tube cavity of the drainage tube, and the tip end of the puncture needle extends out of the drainage tube to be suitable for puncture; and the throttling structure and the drainage tube are arranged in a split mode. When an operation is carried out, a medical worker sleeves the drainage tube on the needle head of the puncture needle, then punctures at a preset position, then punctures the drainage tube into the preset position from one end of the drainage tube along the needle head, then pulls out the needle head, so that aqueous humor flows out from the preset position through the drainage tube, then immediately connects the throttling structure with the drainage tube, throttles the aqueous humor flowing out of the drainage tube through the throttling structure, and controlsthe flow speed of the aqueous humor. Throttling is carried out only through the throttling structure, the medical worker does not need to control the flow speed of aqueous humor, the number of human factors is small, the flow speed of the aqueous humor is relatively stable, operation of the medical worker is simplified, and the aqueous humor drainage operation is more efficient.

Disclosed are a low molecular polypeptide for preventing and treating inflammation, a preparation method and the use thereof and a pharmaceutical composition containing the polypeptide, wherein the polypeptide can penetrate the eye tissue barrier, can maintain a high concentration in neutral tears, aqueous humour and vitreous humour, and can be used to treat inflammation.

The invention provides an aqueous humor analysis method based on a titanium oxide nano-material matrix, which comprises the following specific steps: preparing a material dispersion solution from a titanium oxide nano-material and deionized water in a mass volume ratio range of 1-2 g/L, depositing 1-2 [mu] L of an aqueous humor sample on a target plate, drying the sample, and carrying out vacuum drying on the sample to obtain the titanium oxide nano-material matrix based aqueous humor analysis method based on the titanium oxide nano-material matrix. And applying 1-2 [mu] L of a material dispersion solution on the substrate, carrying out MALDI-TOF-MS mass spectrometry to obtain a metabolic fingerprint spectrum, and distinguishing cataract patients related to different diseases through a machine learning method. According to the method, the metabolic fingerprint spectrum of the aqueous humor sample is acquired by preparing the efficiently ionized binary laser/desorption ionization matrix, and the senile related cataract, the diabetes related cataract and the high myopia related cataract can be quickly distinguished by combining with a machine learning means; the method has a wide application prospect for distinguishing cataract related to different diseases and exploring a cataract molecular mechanism.

The invention discloses a solid-phase multiplex row PCR detection kit and a detection method for infectious eye disease pathogens. The solid-phase multiplex row PCR detection kit comprises a PCR mixedsolution, immobilized primer and probes, a positive control, a negative control and RNase-free water, wherein the immobilized primers and probes are eight herpes virus primers and probes subjected toimmobilization treatment and are separately arranged in triple row PCR tubes, and 2-3 herpes virus primers and different fluorescently labeled probes are arranged in each PCR tube to detect eight herpes viruses simultaneously. The scheme of the invention has high sensitivity and high specificity, primer dimers can be reduced, and the amplification efficiency can be improved; by collecting tear, cornea infected parts, tissues, aqueous humor or vitreous humor and other specimens from the eyes of a patient for detection, disease-associated viruses can be simply, quickly and accurately screened to form etiological diagnosis, and clinical targeted medication can be assisted.

The invention provides an oocyte in-vitro maturation culture method and an application thereof. The method of the invention comprises the step of applying aqueous humor from a non-human animal to in-vitro maturation of oocytes of a non-human mammal. Experiments prove that the oocytes matured by the maturation culture method has remarkably higher developmental rate of subsequent in-vitro fertilized embryos and nuclear transfer embryos than a control group, indicating that the method can effectively improve the maturing quality of the in-vitro oocytes. Aqueous humor is used as a basic culture solution for in-vitro maturation culture of the oocytes and has natural advantages as follows: the aqueous humor is rich in nutritional ingredients to meet various nutritional requirements required by in-vitro maturation of the oocytes firstly; various natural growth factors contained in the aqueous humor play important roles in promoting in-vitro maturation of oocytes and later-stage in-vitro embryonic development secondly; and aqueous humor is directly used for culture, thereby avoiding tedious preparation process of culture solution and facilitating large-scale popularization and application of in-vitro embryo culture thirdly.

The invention discloses a flushing needle for anterior chamber paracentesis. The flushing needle comprises a puncture needle head and a cylinder body. The flushing needle is characterized in that the puncture needle head is nested in a flat needle head with a smooth port, and a push rod is arranged on the outer surface of the cylinder body; a cavity is formed in the outer edge of the cylinder body, and a measuring valve body for measuring a pH value in the cylinder body and a pH adjusting valve body for adjusting the pH value in the cylinder body are arranged in the cavity; and the pH adjusting valve body is used for adjusting a pH value of liquid in the cylinder body according to feedback information of the measuring valve body. Firstly, the puncture needle head penetrates through the inner side of the limbus corneae of a patient to form a puncture opening, then, the push rod is used for enabling the flat needle head to slide and wrap the puncture needle head, and then, the flat needle head is used for flushing an anterior chamber; the pH value of the liquid in the cavity is obtained through the measuring valve body in the cavity, and the pH value of the liquid in the cavity is equal to that of the liquid in the anterior chamber; and the pH value in the cylinder body is adjusted by using the pH adjusting valve body, so that imbalance of the pH value of the anterior chamber caused by replacement of aqueous humor of the anterior chamber and the flushing liquid is avoided.

The invention relates to gene detection by using aqueous humor cfDNA to assist clinical diagnosis, and belongs to the technical field of gene detection. The cfDNA in aqueous humor is used for detection, obtained mutation information is compared with a reference genome, primary vitreoretinal lymphoma (PVRL) can be effectively diagnosed, the detection result is significantly better than the detection accuracy of the cfDNA in plasma, and early diagnosis can be achieved.

The invention relates to the technical field of orthokeratology, and discloses a preparation method of an aspheric optical zone of an orthokeratology lens. The method comprises the steps of: determining a human eye mathematical model which comprises a cornea, aqueous humor, a pupil, a crystalline lens, a vitreous body and a retina; performing optical simulation according to the human eye mathematical model and basic simulation parameters; and preparing the aspheric optical area of the orthokeratology lens based on a simulation result of optical simulation. The method for designing the aspheric optical zone of the orthokeratology lens is controllable, clear in theoretical path and guided by practical indexes, the human eyes are adjusted and corrected to be stable and controllable myopia defocus on the premise that the visual quality is guaranteed during use, the effect of preventing the eye axes of the human eyes from increasing is expected to be achieved, and therefore, the function of controlling the myopia progress of the human eyes is improved.