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Micro-fluidic cell culture device for simulating filtering bubble and application of micro-fluidic cell culture device

A technology of cell culture and filtration bleb, which is applied in tissue cell/virus culture device, enzymology/microbiology device, biochemical cleaning device, etc. It can solve the problem that it is difficult to reflect the function of the filter bleb and cannot simulate the three-dimensional filter bleb Problems such as the fluid field and the inability to realize the microfluidic circulation of the culture medium

Pending Publication Date: 2022-04-19
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the cellular level, choosing 2D culture is also difficult to reflect the function of the filter bleb, and it is difficult to simulate the filter bleb
It is a dynamic process for aqueous humor to reach the filtering bleb and then enter the circulatory system. Conventional static in vitro 2D cell culture can neither simulate the fluid field in the three-dimensional filtering bleb nor realize the microfluidic circulation of the culture medium.

Method used

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  • Micro-fluidic cell culture device for simulating filtering bubble and application of micro-fluidic cell culture device
  • Micro-fluidic cell culture device for simulating filtering bubble and application of micro-fluidic cell culture device
  • Micro-fluidic cell culture device for simulating filtering bubble and application of micro-fluidic cell culture device

Examples

Experimental program
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Effect test

Embodiment 1

[0078] This embodiment provides a microfluidic cell culture device for simulating filtration blebs, the vertical cross-sectional view of which is parallel to the center of the long side through the injection hole 4 is shown in figure 2 As shown, the horizontal cross-section is shown as image 3 shown.

[0079] The microfluidic cell culture device for simulating filtration blebs comprises a cover plate 1, a cell culture body 2 and a grooved bottom plate 3 containing microchannels from top to bottom;

[0080] The cell culture main body 2 contains a cell culture cavity 7 with a flat bottom and a semi-arc dome-like bulge;

[0081] The grooved bottom plate 3 is engraved with an inflow channel 6, an outflow channel 8 and a collective outflow channel 10;

[0082] The number of the inflow channel 6 is one, which is connected to the short arc edge of the cell culture chamber 7;

[0083] The cross-section of the inflow channel 6 is a rectangle, the length of the long side of the rec...

Embodiment 2

[0091] This embodiment provides a microfluidic cell culture device for simulating filtration blebs, the vertical cross-sectional view of which is parallel to the center of the long side through the injection hole 4 is shown in figure 2 As shown, the horizontal cross-section is shown as image 3 shown.

[0092] The microfluidic cell culture device for simulating filtration blebs comprises a cover plate 1, a cell culture body 2 and a grooved bottom plate 3 containing microchannels from top to bottom;

[0093] The cell culture main body 2 contains a cell culture cavity 7 with a flat bottom and a semi-arc dome-like bulge;

[0094] The grooved bottom plate 3 is engraved with an inflow channel 6, an outflow channel 8 and a collective outflow channel 10;

[0095] The number of the inflow channel 6 is one, which is connected to the short arc side of the cell culture chamber 7;

[0096] The cross-section of the inflow channel 6 is a rectangle, the length of the long side of the rec...

Embodiment 3

[0104] This embodiment provides a microfluidic cell culture device for simulating filtration blebs, the vertical cross-sectional view of which is parallel to the center of the long side through the injection hole 4 is shown in figure 2 As shown, the horizontal cross-section is shown as image 3 shown.

[0105] The microfluidic cell culture device for simulating filtration blebs comprises a cover plate 1, a cell culture body 2 and a grooved bottom plate 3 containing microchannels from top to bottom;

[0106] The cell culture main body 2 contains a cell culture cavity 7 with a flat bottom and a semi-arc dome-like bulge;

[0107] The grooved bottom plate 3 is engraved with an inflow channel 6, an outflow channel 8 and a collective outflow channel 10;

[0108] The number of the inflow channel 6 is one, which is connected to the short arc side of the cell culture chamber 7;

[0109] The cross-section of the inflow channel 6 is a rectangle, the length of the long side of the rec...

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Abstract

The invention provides a micro-fluidic cell culture device for simulating filter bubbles and application of the micro-fluidic cell culture device. The micro-fluidic cell culture device for simulating the filter bubbles comprises a cover plate, a cell culture main body and a notch groove bottom plate containing a micro-channel from top to bottom, the cell culture main body contains a semi-arc dome-shaped raised cell culture cavity with a flat bottom; and an inflow channel, an outflow channel and a gathering outflow channel are engraved on the grooving bottom plate. The invention also provides a use method of the micro-fluidic cell culture device for simulating the filtering bubble. The micro-fluidic cell culture device for simulating the filtering bubble simulates the biological form of the filtering bubble and the one-way and dynamic flowing process of aqueous humor in eyes, can be used for culturing cells in a state close to a natural physiological state and researching the functions of the cells, and has important significance on research of related diseases.

Description

technical field [0001] The invention belongs to the technical field of in vitro cell culture devices, and in particular relates to a microfluidic cell culture device for simulating filtration blebs and its application. Background technique [0002] Glaucoma is an irreversible blinding eye disease with the highest incidence worldwide, and its pathogenesis is that pathological high intraocular pressure causes damage to the optic nerve. Since the 1960s, trabeculectomy has been widely used in the treatment of glaucoma, becoming an effective way to reduce intraocular pressure and the gold standard of anti-glaucoma surgical treatment. [0003] Trabeculectomy aims to properly drain the aqueous humor in the eye to the scleral pool by removing part of the trabecular network tissue, thereby reducing pathological high intraocular pressure, and finally the aqueous humor accumulates between the fascia tissue and the sclera tissue to form filter bleb. The aqueous humor in the filtration...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36
CPCC12M23/16C12M41/40
Inventor 殷雪王强斌陆培荣
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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