Extraction method and kit of aqueous humor cfDNA and application of aqueous humor cfDNA in PVRL clinical auxiliary examination

An extraction method and kit technology, applied in the field of genetic testing, can solve problems such as difficult sampling and difficult diagnosis, and achieve the effect of early diagnosis

Active Publication Date: 2021-09-17
GENESEEQ TECH INC +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is: in the diagnosis process of primary vitreoretinal lymphoma (PVRL), there are similar clinical symptoms and great difficulty in diagnosis; Vitreous sampling has the problem of difficult sampling

Method used

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  • Extraction method and kit of aqueous humor cfDNA and application of aqueous humor cfDNA in PVRL clinical auxiliary examination
  • Extraction method and kit of aqueous humor cfDNA and application of aqueous humor cfDNA in PVRL clinical auxiliary examination
  • Extraction method and kit of aqueous humor cfDNA and application of aqueous humor cfDNA in PVRL clinical auxiliary examination

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Embodiment

[0047] Example PVRL patient aqueous humor cfDNA detection

[0048] Since the composition of aqueous humor is similar to that of plasma and fills the anterior and posterior chambers of the eyeball, sampling is noninvasive. Select aqueous humor samples to explore whether they are suitable as liquid biopsy samples for PVRL patients, and select aqueous humor samples from 4 PVRL patients in the above comparative example for testing to verify whether the aqueous humor cfDNA can reflect the patient's gene mutation status.

[0049] The nucleic acid extraction kit QIAamp Circulating Nucleic Acid Kit (QIAGEN) was used to extract the cfDNA of the aqueous humor sample, and then the library was constructed, enriched, and quantified, and then the Illumina HiSeq next-generation sequencing system was used. Through medical analysis, it was found that the aqueous humor samples of the 4 patients in the comparative example were all positive (Table 2). The total number of mutations detected in th...

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Abstract

The invention relates to gene detection by using aqueous humor cfDNA to assist clinical diagnosis, and belongs to the technical field of gene detection. The cfDNA in aqueous humor is used for detection, obtained mutation information is compared with a reference genome, primary vitreoretinal lymphoma (PVRL) can be effectively diagnosed, the detection result is significantly better than the detection accuracy of the cfDNA in plasma, and early diagnosis can be achieved.

Description

technical field [0001] The invention relates to gene detection using aqueous humor cfDNA to assist clinical diagnosis, and belongs to the technical field of gene detection. Background technique [0002] In recent years, the global incidence and mortality of cancer have been increasing, and cancer has become a major public health problem worldwide. In 2020, it is estimated that there will be nearly 19.3 million new cancer cases (10.07 million men and 9.23 million women) worldwide, and about 10 million cancer deaths. Breast cancer has replaced lung cancer as the most common cancer, accounting for nearly 12% of new cases worldwide each year. As a complex and diverse disease, cancer has great heterogeneity in molecular genetics. Therefore, it is very important for individualized and precise diagnosis and treatment of cancer patients. Genetic testing is of great significance in early cancer screening, auxiliary cancer diagnosis / differential diagnosis, guidance of targeted thera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6806
CPCC12Q1/6886C12Q1/6806C12Q2600/156C12Q2527/125C12Q2521/537
Inventor 刘璟文闫重光邵阳史其萍汪笑男孙小慧孙泽华
Owner GENESEEQ TECH INC
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