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Autologous adipose-derived mesenchymal stem cell culture method and used culture medium

A technique of stromal stem cells and a culture method, which is applied to the culture medium used in the culture of the above-mentioned autologous adipose mesenchymal stem cells, autologous adipose mesenchymal stem cells, and autologous adipose mesenchymal stem cells, can solve the problem of low cell viability, Problems such as low cell yield

Active Publication Date: 2017-11-24
广东博雅干细胞科技有限公司
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Problems solved by technology

[0012] Although the prior art discloses some methods for culturing adipose-derived mesenchymal stem cells such as the above, the inventors have found that these methods do not seem to be applicable to eye fat, such as adipose-derived mesenchymal stem cells related to eye bags / eyelids, such as these The method has low cell yield and / or low cell viability when used to obtain adipose-derived mesenchymal stem cells from ocular fat

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  • Autologous adipose-derived mesenchymal stem cell culture method and used culture medium

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Embodiment 1

[0254] Example 1: Culturing eye fat mesenchymal stem cells and preparing eye fat mesenchymal stem cell preparations

[0255] (1) Culture pretreatment: transfer the eye fat collected from the eye bag to a biological safety cabinet, pour it into a petri dish containing PBS containing penicillin and streptomycin, use sterile surgical scissors and sterile tweezers to remove the fat on the fat. Vascular and connective tissue, and adipose tissue were washed with PBS.

[0256] (2) Adhesive culture: adipose tissue into 4-5mm 3 Take a clean 10cm petri dish, use tweezers to paste the fat fragments in the petri dish, stick 10-15 pieces to a petri dish, dry it in a biological safety cabinet for about 30min, add 10ml to each petri dish Culture medium (the adipose tissue should be prevented from floating when adding. If there is adipose tissue floating, you can cover it with a sterile coverslip to help it adhere to the wall).

[0257] (3) Rehydration and liquid replacement: rehydration ...

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Abstract

The invention relates to an autologous adipose-derived mesenchymal stem cell culture method and a used culture medium. Specifically, the culture method disclosed by the invention comprises the following steps of culture pretreatment, adherent culture, fluid replacement and fluid change, one time or optional multiple times of cell passage, optionally preparing an adipose-derived mesenchymal stem cell preparation and optionally performing cell cryopreservation and revival on the obtained adipose-derived mesenchymal stem cells. The the culture medium disclosed by the invention comprises DMEM and FBS. When the culture medium disclosed by the invention is utilized to culture the autologous adipose-derived mesenchymal stem cells, especially the autologous adipose-derived mesenchymal stem cells of eye adipose cells, an excellent technological effect of the culture method disclosed by the invention can be presented; the obtained autologous adipose-derived mesenchymal stem cells can be applied to autologous application; for example, the autologous adipose-derived mesenchymal stem cells obtained from eye adipose can be applied to face injection such as injection filling at parts of face wrinkles, temple and lacrimal sulcus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for culturing adipose-derived mesenchymal stem cells, in particular to a method for culturing autologous adipose-derived mesenchymal stem cells, wherein the autologous adipose-derived mesenchymal stem cells are especially derived from autologous Adipose-derived mesenchymal stem cells. The present invention also relates to the medium used in the above-mentioned culture of autologous adipose-derived mesenchymal stem cells. When the medium of the present invention is used to culture the autologous adipose-derived mesenchymal stem cells, especially the autologous adipose-derived mesenchymal stem cells of eye fat cells, the excellent technical effect of the present invention can be exhibited. The obtained autologous adipose-derived mesenchymal stem cells can be used for autologous applications. For example, the autologous adipose-derived mesenchymal stem cells obtained from eye fat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2501/998C12N2509/00
Inventor 李诣书周丹王正
Owner 广东博雅干细胞科技有限公司
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