Use of a combination of specific lectins in the preparation of a test vector for identifying epithelial ovarian cancer and a kit

A lectin and ovarian cancer technology, applied in biological testing, measuring devices, instruments, etc., can solve problems such as low specificity, hazards of blood-borne diseases in samples, and unsuitability for large-scale population censuses, and achieve high-throughput detection, Effects that are easy to capture

Active Publication Date: 2019-12-03
深圳格道糖生物技术有限公司
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  • Abstract
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Problems solved by technology

[0009] In order to solve the current problems in the differential diagnosis of epithelial ovarian cancer, such as low specificity, hazards of blood-borne diseases in samples, and unsuitability for large-scale population census, etc.

Method used

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  • Use of a combination of specific lectins in the preparation of a test vector for identifying epithelial ovarian cancer and a kit
  • Use of a combination of specific lectins in the preparation of a test vector for identifying epithelial ovarian cancer and a kit
  • Use of a combination of specific lectins in the preparation of a test vector for identifying epithelial ovarian cancer and a kit

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Embodiment Construction

[0032] The present invention will be further illustrated by the specific examples given below, but it is not intended to limit the present invention.

[0033] In the present invention, 37 lectins (shown in Table 1) are screened for lectin probes for differential diagnosis of female patients with epithelial ovarian cancer.

[0034] Table 1

[0035]

[0036]

[0037]

[0038] The kit materials used in the present invention include: protease inhibitor, DMSO, Cy3, Cy5 fluorescent dyes, Sephadexg G-25 column, bovine serum albumin (BSA), glass substrate, oxysilane reagent (GPTS), Bradford reagent , PVDF membrane, Tween-20.

[0039] 1. Collection of Saliva Samples

[0040] A total of 192 saliva samples were collected, including 63 healthy female volunteers (HV) saliva, 51 saliva patients with benign ovarian disease (OB), and 78 diagnosed epithelial ovarian cancer (OC) patients, including 24 stage I patients. , 22 patients with stage II, and 32 patients with stage III. As...

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Abstract

The present invention proposes the use of a specific lectin combination in preparing test vectors for identifying epithelial ovarian cancer and a kit. For saliva samples, the specific lectin combinations are: (1) the combination of PSA, SNA, MAL‑II, ECA and HHL, corresponding to healthy people; (2) the combination of PHA‑E, LTL and PNA, corresponding to benign Patients with ovarian disease; (3) The combination of RCA120, UEA‑I, ConA, LCA, AAL, WFA, WGA, DBA, VVA, NPA and GNA, corresponding to patients with epithelial ovarian disease. By specifically binding lectins to glycoprotein sugar chains in saliva, differentially expressed glycoproteins and sugar chain structures in patient saliva samples can be quickly detected, achieving rapid and high-performance detection of multiple glycoproteins and sugar chain structures in the samples. Flux detection.

Description

technical field [0001] The present invention relates to a lectin application and a kit for identifying epithelial ovarian cancer based on sialic glycoprotein. Background technique [0002] Ovarian cancer (OC) is one of the common female reproductive system tumors, ranking the sixth among the common cancers in the world, the fifth among the common female cancers, and the seventh among the main cancers leading to the death of women worldwide. . In recent years, the incidence of ovarian cancer has been on the rise in my country. It is estimated that the incidence of ovarian cancer in my country will reach 6.43 / 100,000 in 2016. Due to the lack of obvious distinguishing signs and screening methods, early diagnosis of ovarian cancer is very difficult. Clinical studies have shown that the 5-year survival rate of ovarian cancer patients diagnosed in early stage (I / II) can reach more than 90%, however, about 70%-85% of patients are in advanced stage (stage III / IV) at the time of dia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57449G01N33/68
Inventor 李铮薛添张坤舒健刘夏薇阳佳君于汉杰杜昊骐
Owner 深圳格道糖生物技术有限公司
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