Monoclonal cell culture method
A cell culture and monoclonal technology, which is applied in the direction of cell culture support/coating, tissue culture, biochemical equipment and methods, etc., can solve the problems affecting the experimental operation, few single cells, and undivided cells, etc., to improve survival rate effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0079] The monoclonal cell culture method provided in this application is suitable for use in the process of constructing stable expression cell lines using lentiviral transfection system and gene editing technology. This example is based on the CRISPR / Cas9 gene editing technology, taking knockout of the IDH1 gene (isocitrate dehydrogenase-1 gene) in the glioma 293T cell line and U87 cell line as an example, and compared the existing common culture The system and the growth difference of single cell clones in the 3D soft fibrin matrigel culture system of this application are briefly introduced as follows.
[0080] (1) According to the existing technology, use CRISPR / Cas9 technology to edit the genome, specifically: design the sgRNA sequence targeting IDH1, and design the reporter sequence that can be used to screen the sgRNA with higher activity according to its sequence ( reporter gene sequence); construct the recombinant plasmid IDH1-sgRNAs-PX330 and the reporter gene plasmi...
PUM

Abstract
Description
Claims
Application Information

- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com