Method for promoting growth of mice liver implanted tumor after partial hepatectomy

A tumor growth and mouse technology, applied in the field of promoting the growth of mouse liver implanted tumors, to achieve the effect of promoting growth

Inactive Publication Date: 2018-06-12
THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for promoting growth of mice liver implanted tumor after partial hepatectomy
  • Method for promoting growth of mice liver implanted tumor after partial hepatectomy
  • Method for promoting growth of mice liver implanted tumor after partial hepatectomy

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Embodiment 1

[0036] Example 1 Method for Promoting the Growth of Mouse Hepatic Implanted Tumors After Partial Hepatectomy

[0037] 1 Materials and methods

[0038] 1.1 Liver cancer cell lines and experimental animals

[0039] In this study, H22 mouse liver cancer cell line was used, and 4-week-old male C57BL / 6J mice were used as inoculated animals for intraperitoneal culture. 4-week-old male BALB / c nu nude mice with a body weight of about 20-25 g were used as mice for the preparation of scapular implant tumor and liver implant tumor models. The above cell lines and mice were purchased from the Animal Experiment Center of Sun Yat-Sen University East Campus, and the experimental site was at the Animal Experiment Center of Sun Yat-Sen University North Campus.

[0040] The procedures for all animal experiments were approved by the Institutional Animal Care and Use Committee at The Third Affiliated Hospital of SunYat-Sen University.

[0041] 1.2 Reagent materials

[0042] RPMI1640 low-gluco...

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Abstract

The invention relates to a method for promoting the growth of mice liver implanted tumor after partial hepatectomy. The method comprises the following steps: culturing a mice liver cancer cell line, establishing a nude mice scapular region implanted tumor, establishing a mice different-ratio liver resection model, and establishing a mice liver implanted tumor model; and inoculating a tumor tissueblock after the parital hepatectomy, and injecting an Hh signal inhibitor every day after the inoculation. The scheme of the invention proves that the liver regeneration Hh signal channel after the hepatectomy can also promote the growth of the liver implanted tumor, prompts that the Hh signal channel can become a novel target point of the reoccurrence and transfer of the liver cancer, and can beused for further researching the effect of the Hh signal channel for preventing the reoccurrence of the liver cancer. The result of the invention has important significance for researching the reoccurrence prevention after the hepatectomy.

Description

technical field [0001] The invention relates to the technical field of medicine and disease treatment, in particular to a method for promoting the growth of mouse hepatic tumors after partial hepatectomy. Background technique [0002] Liver cancer is the fourth most common malignant tumor and the third most common lethal tumor worldwide. Multidisciplinary comprehensive treatment is currently the best treatment model for liver cancer. Radical hepatectomy is the best treatment option for early and mid-stage HCC with normal liver function reserve. However, more than 80% of patients undergoing radical resection of liver cancer relapse within 5 years after operation, seriously affecting the long-term survival rate of patients. The recurrence after radical resection of liver cancer is closely related to multicentric tumor growth and intrahepatic tiny tumor thrombi. [0003] When the apoptosis rate of tumor cells is balanced with the proliferation rate, tumor micrometastases wil...

Claims

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Application Information

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IPC IPC(8): A61K35/13A61K31/58A61P35/00
CPCA61K31/58A61K35/13
Inventor 汪根树王其友张俊斌李慧谌小龙谢俊强李学俊刘慧玲周静冯智英王励
Owner THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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