Step reconstruction and counting method for leukocyte and platelet coexistence hologram

Abstract

The invention relates to a step reconstruction and counting method for a leukocyte and platelet coexistence hologram, and belongs to the field of medical application of a digital holographic imaging technology. The method comprises the steps of firstly performing preliminary convolutional reconstruction on the leukocyte and platelet coexistence hologram, extracting regions corresponding to the leukocytes through a wavelet decomposition coefficient, improving region capture accuracy by using a morphological means and performing counting; secondly performing inverse diffraction on region reconstruction information to restore a corresponding diffraction ring, and performing secondary reconstruction after counteraction with a diffraction ring in the original hologram, thereby obtaining clear images of the platelets; and finally performing good identification and accurate counting on the platelets through a grayscale threshold obtained by grayscale expansion and logarithmic normal distribution fitting. In addition, the method is suitable for hologram reconstruction of not only the leukocytes and the platelets but also other coexistent objects with remarkable volume difference, and has more extensive meaning.

Description

technical field [0001] The invention belongs to the medical application field of digital holographic imaging technology, and relates to a step-by-step reconstruction and counting method for coexisting holograms of white blood cells and platelets. Background technique [0002] Blood is an indispensable part of the human body. The normality of various cells and contents in the blood is directly related to the health of the human body. Routine blood test is a common test item in medical treatment. With the advancement of current detection technology, the methods and means that can be used for blood routine detection are more and more abundant: [0003] ①Microscopic counting method: [0004] Principle: Using manual microscopy (mostly using cell counting board, also known as Niu Bao board), with the help of dyes, contrast agents or fluorescent dyes, medical examiners can directly observe, mark, classify and count various types of blood cells. [0005] Advantages: This method i...

AI Technical Summary

Problems solved by technology

[0006] Disadvantages: manual detection is costly and inefficient, and cannot meet medical needs; limited by the narrow field of view of the microscope, it is impossible to observe and count large blood samples at the same time; dyeing is required, causing irreversible damage to cells

[0010] Disadvantages: The equipment is bulky, expensive, not portable, and the internal structure is complex and difficult to miniaturize; the resolution and processing ability for cell backflow, wandering between wells, adhesion, etc. is not good

[0014] Disadvantages: expensive, high equipment maintenance costs, complicated blood sample flow path and complex optical path in the instrument

[0018] Disadvantages: high requirements on sample and image quality, cumbersome pretreatment of samples is often required; limited by the field of view when the microscope captures images, the amount of sample included is low, and the introduction of accidental errors is large

Therefore, when the hologram is reconstructed by conventional reconstruction methods, the morphology information of white blood cells is well preserved, while the information of platelets is too weak to be covered up. After direct reconstruction, the imaging results of platelets are difficult or impossible to extract, so it cannot help Experiments and inspection personnel obtain useful information

Images