A detection method for full-band biological chromogenic experiment

Abstract

The invention discloses a detection method of a full-wave band biological color development experiment, and belongs to the field of biological detection. According to the detection method, a detectionsystem is arranged and comprises a shooting unit, an input unit and an analysis processing unit, wherein the shooting unit is used for acquiring the images of the biological color development experiment and extracting the absorption value of the sample in the images in the red green and blue three-color passage; then, the input unit inputs the sample standard product concentration range into theanalysis processing unit for standardized calibration. The analysis processing unit is used for performing the analysis calculation on the input sample absorption value and the standard product concentration range; the concentration value of the sample to be tested is finally output. The algorithm of the method can be easily mastered; the performance is stable; the precision is high; the operationtime is short; the detection can be performed by aiming at the full-wave band biological color development experiment.

Description

technical field [0001] The invention relates to a biological detection method, in particular to a detection method for a full-band biological chromogenic experiment. Background technique [0002] Biological chromogenic experiments include enzyme-linked immunosorbent assay, bicinchoninic acid assay, Bradford protein assay and Lowry protein assay. Biochromogenic assay is one of the methods for detecting protein biomarkers, which is widely used in early disease diagnosis, and it can detect diseases such as cardiovascular disease, Alzheimer's disease and rheumatoid arthritis. [0003] The paper "Appropriate Calibration Curve Fitting in Ligand Binding Assays" (9, 260-267, AAPS Journal, 2007) introduces a conventional bioassay method. This technique requires the use of a microplate reader based on an optical graduation to measure the absorbance of the sample solution. The absorbance of the reaction product is precisely selected with light of a specific wavelength through a diffr...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems of complex fitting models, long operation time and single detection band in the current biological detection technology, and proposes a detection method for full-band biological chromogenic experiments, which has the advantages of simple model, short calculation time, and full-band coverage , the advantages of high measurement accuracy

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