Application of nanoporous microscaffolds in tissue regeneration and repair
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A nano-porous, tissue regeneration technology, applied in the field of regenerative medicine, can solve the problems of undeveloped stem cell survival, expansion, directed differentiation, injectable three-dimensional porous micro-scaffold, achieve small physical size, high connectivity rate, and promote adherence Effect
Active Publication Date: 2019-11-05
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[0008] However, injectable three-dimensional porous microscaffolds that can effectively promote stem cell survival, expansion, and directed differentiation have not yet been developed.
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Embodiment 1
[0124] Example 1 Preparation of nano-carrier-sodium alginate three-dimensional porous micro-scaffold
[0125] 1.1. Synthesis of peptide-laden mesoporous silicananoparitcles (pep@MSNs)
[0126] Mesoporous silica nanoparticles (MSNs) come from the Wei Jie laboratory of East China University of Science and Technology. The specific preparation method is as follows: Add 3-9g Pluronic P-123 (Sigma), 125-130mL deionized water and 18-25mL concentrated HCl into a round bottom flask, stir vigorously for 1.5-4h, and set the flask at 50℃ after mixing thoroughly. In a water bath, add 5-10 g of ethyl orthosilicate with stirring, and stir for 20 hours. Then the temperature is raised to 80-85°C, and the temperature is kept constant for 24-30h; then it is centrifuged (5000-6000G), the precipitate is washed with deionized water, dried and then calcined in a muffle furnace.
[0127] In this study, the peptide we used is a peptide derived from the immature region of bone morphogenetic protein-7, name...
Embodiment 2
[0139] Example 2 Characterization of nano-carrier-sodium alginate three-dimensional porous microscaffold
[0140] Scanning electron microscope (SEM; S-4800; Hitachi, Japan) is used to characterize the microstructure (pore size, porous morphology, etc.) of AS, AMS and NAC / MS and the size of the micro-scaffold; compressive elastic modulus tester (ElectroForce 3100; Bose , USA) is used to determine the elastic modulus of each stent.
[0141] Soak the freeze-dried micro-scaffold in PBS, wait for it to fully absorb water and swell, filter out the PBS, add a small amount of low-concentration sodium alginate solution to the micro-stent and mix well, then transfer to a syringe (1mL specification) to freeze-dry . For in vivo experiments, only need to add the cell suspension to the micro-scaffold and mix thoroughly before injection. Dense tissue is used to verify its injectability.
[0142] The sustained release characteristics of BFP-1 coated in NAC / MS were characterized by measuring the ...
Embodiment 3
[0150] Example 3 Preparation of injectable NAC / MS
[0151] Soak the prepared NAC / MS in PBS at 4°C for 48h; then filter out the PBS, transfer the NAC / MS to a 1ml syringe, plug the injection piston, freeze-dry and store in a sealed container at 4°C.
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Abstract
The invention discloses an application of a nano porous microbracket in tissue regeneration and repair. The nano porous microbracket is a nano carrier-sodium alginate three-dimensional porous microbracket; the nano carrier is mesoporous silicon dioxide nanoparticles; the sodium alginate is modified sodium alginate; and the nano carrier-sodium alginate three-dimensional porous microbracket is prepared by adopting a liquid nitrogen quick freezing high speed breaking method. The special application method of the nano carrier-sodium alginate three-dimensional porous microbracket in tissue regeneration and repair comprises the following steps: adding PBS which accounts for 50-80% of the total volume of the microbracket into a prepared microbracket to obtain an injectable microbracket; and injecting the injectable microbracket to a damaged tissue part. The nano porous microbracket can promote tissue regeneration and promotes cell amplification and oriented differentiation of stem cells, is injectable and is high in clinical practicality. The invention also discloses applications of the nano porous microbracket in promoting stem cell proliferation, stem cell differentiation and tissue self-regeneration and repair.
Description
Technical field [0001] The invention relates to an application of a scaffold in tissue regeneration and repair, belonging to the field of regenerative medicine, in particular to the application of a nano porous micro scaffold in tissue regeneration and repair. Background technique [0002] Biological scaffolds can effectively promote the self-renewal, proliferation and directed differentiation of stem cells by simulating the physical and chemical properties of the human body's natural microenvironment. In recent years, it has been widely used in the field of regenerative medicine. [0003] Generally, stem cell-based biological scaffolds are mainly divided into porous scaffolds and hydrogel scaffolds according to their states. Among them, the porous scaffold has a large number of interconnected three-dimensional porous structures, which is more conducive to the migration of cells in the scaffold and the supply of nutrients than hydrogels. It is generally believed that the three-dim...
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