Variation malus spectabilis tissue culture and breeding method

Abstract

The invention discloses a bonsai-type ornamental plant variation malus spectabilis tissue culture and breeding method. The method includes first treating a variation malus spectabilis plant with gibberellin, cutting a newly-extracted branch as an explants, washing and disinfecting, cutting a stem segment of 1-3cm, culturing the stem segment on a culture medium 1 / 2MS+6-BA0.2mg / L+0.1g / L activated carbon, grafting the selected lateral buds to a first medium 1 / 2MS+6-BA5mg / L, conducting subculture for 20 days and then grafting to a second medium 1 / 2MS+6-BA1.0mg / L, conducting culture for 20 days, then grafting to a third medium MS+6-BA0.4mg / L, conducting culture for 25 days, grafting the twig with the length of 2cm or more to a rooting medium 1 / 2MS+0.5IBA for culture, and finally forming a matrix according to the ratio of peat: perlite = 1:1, and covering a film and a shading net for refining a seedling. The obtained variation malus spectabilis tissue culture seedling has the rooting percentage of 95% or more, and the breeding speed is fast.

Description

technical field [0001] The invention belongs to the field of plant tissue breeding, and particularly relates to a tissue culture and breeding technology of bonsai ornamental plant winter red fruit, in particular to a tissue culture breeding method of variant winter red fruit. Background technique [0002] Donghongguo belongs to the Rosaceae apple genus. The flowers are light pink. They open in spring and bear fruit immediately. The fruit matures in autumn. It does not fall until February to March of the second year. The ripe fruit weighs 10 to 20g, and the single ripe fruit is spherical or oblate. At the same time, the plant of winter red fruit is short and compact, so the ornamental value is extremely high. [0003] At present, one of the most commonly used techniques in plant production is the rapid propagation of excellent varieties through tissue culture. Tissue culture methods generally include explant selection and disinfection, medium culture, rooting culture, and see...

AI Technical Summary

Problems solved by technology

[0005] In order to maintain the specificity of the mutated Donghong fruit, when using conventional tissue culture techniques to breed the mutated Donghong fruit, the obtained seedlings have obvious character separation, and the excellent specific characters of the mutated Donghong fruit cannot be stably maintained. Therefore, in the field There is an urgent need to solve the difficult problem of tissue culture and breeding of mutated Donghong fruit, so that the specific characters of mutated Donghong fruit can be stably maintained, and the fruiting rate should also be improved.