An all-fiber sted super-resolution micro-illumination device

Abstract

The present invention provides an all-fiber STED super-resolution microscopic illumination device, which is mainly composed of a laser 1 , a delay unit 2 and a beam generator 3 . Wherein, the beam generator 3 is mainly composed of a section of double-clad optical fiber 4 and a polarization controller 5 . The beam generator 3 can convert the short-wave laser light 6 and long-wave laser light 7 emitted by the laser 1 into Gaussian excitation light 8 and hollow ring loss light 9 respectively, because the spot centers of these two beams of light coincide completely, and the latter passes through After the delay of the delay unit 2, the two beams of light are irradiated onto the fluorescent sample 10 successively, and stimulated emission depletion (STED) occurs, and the fluorescent light 11 with a very small spot is excited, and the final resolution is no longer limited by the diffraction of light. Thereby breaking the diffraction limit and realizing all-fiber STED super-resolution microscopic illumination. The invention has the advantage that the beam generator is highly integrated, making the equipment more flexible and stable.

Description

[0001] (1) Technical field [0002] The invention relates to the fields of fiber optic technology and optical microscope technology, in particular to an all-fiber stimulated emission depletion (STED) super-resolution microscopic illumination imaging device. [0003] (2) Background technology [0004] The development of modern biology and materials science has put forward higher and higher resolution requirements for microstructure research. Stimulated emission depletion (STED) microscopy is a super-resolution microscopy based on laser focusing microscopy, and its imaging speed is relatively fast, which provides a new method for research in the fields of biomedicine and materials. In the STED microscopic imaging device, two laser beams are required for illumination. One laser beam forms an Airy disk at the focal point of the objective lens, which excites the fluorescent substance and emits fluorescence; the other beam forms a ring with zero central light intensity at the focal p...

AI Technical Summary

Problems solved by technology

For example, the Chinese patent CN211817464U proposes a super-resolution fluorescence microscopy device based on tangential polarization. Although a good microscopic illumination effect is obtained, the realization of this effect requires complex optical paths, expensive precision instruments, and the stability is not very high.

Chinese patent CN103617330A proposes a super-resolution STED microscopic imaging device excited by a broadband laser light source generated by a supercontinuum. Although the function of synchronous adjustment of the two lasers is improved to achieve good microscopic illumination, the overall optical path is complicated

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